Dose-Dependent Effects of Genistein and Daidzein on Protein Metabolism in Porcine Myotube Cultures

• Published in: Journal of agricultural and food chemistry Vol. 57; no. 3; pp. 852 - 857
• Main Authors: Rehfeldt, Charlotte, Kalbe, Claudia, Nürnberg, Gerd, Mau, Marcus
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 11.02.2009
• Subjects: Animals; Animals, Newborn; Bioactive Constituents; bioactive properties; Biological and medical sciences; cell differentiation; cell lines; Cells, Cultured; daidzein; Dose-Response Relationship, Drug; Estradiol - administration & dosage; Food industries; Fundamental and applied biological sciences. Psychology; genistein; Genistein - administration & dosage; Isoflavones - administration & dosage; Muscle Fibers, Skeletal - drug effects; Muscle Fibers, Skeletal - metabolism; Muscle Proteins - drug effects; Muscle Proteins - metabolism; myocytes; myotubes; protein content; protein metabolism; protein synthesis; skeletal muscle; Swine; Isoflavone; differentiation; skeletal muscle; porcine myoblast; estrogen receptor; Genistein; Estrogen; Metabolism; Flavonoid; Ovarian hormone; Protein; Phytoestrogen; Polyphenol; Muscle; Daidzein; Sex steroid hormone; Differentiation
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf803039b

This study was conducted to investigate whether the isoflavones genistein and daidzein, which are components of soy-based diets, and the estrogen 17β-estradiol affect differentiation and protein metabolism of porcine skeletal muscle cells in vitro. Serum-free porcine myotube cultures expressing the estrogen receptors ERα and ERβ were treated with various concentrations of genistein, daidzein, or 17β-estradiol for 26 h. The degree of differentiation by creatine phosphokinase activity was not altered by treatment. At 100 μmol/L both genistein and daidzein caused decreases in protein amount due to cell loss. In addition, 100 μmol/L genistein reduced protein synthesis rate of the surviving cells (P < 0.05) measured as [3H]-phenylalanine incorporation. Interestingly, genistein (0.1 μmol/L), daidzein (10, 100 μmol/L), and 17β-estradiol (0.1, 1 nmol/L) slightly reduced protein degradation (P < 0.05). The results suggest that both genistein and daidzein affect protein metabolism in a dose-dependent manner and that estrogenic actions may play a role in decreasing protein degradation in porcine skeletal muscle.