Dose-Dependent Effects of Genistein and Daidzein on Protein Metabolism in Porcine Myotube Cultures

This study was conducted to investigate whether the isoflavones genistein and daidzein, which are components of soy-based diets, and the estrogen 17β-estradiol affect differentiation and protein metabolism of porcine skeletal muscle cells in vitro. Serum-free porcine myotube cultures expressing the...

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Published inJournal of agricultural and food chemistry Vol. 57; no. 3; pp. 852 - 857
Main Authors Rehfeldt, Charlotte, Kalbe, Claudia, Nürnberg, Gerd, Mau, Marcus
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 11.02.2009
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Summary:This study was conducted to investigate whether the isoflavones genistein and daidzein, which are components of soy-based diets, and the estrogen 17β-estradiol affect differentiation and protein metabolism of porcine skeletal muscle cells in vitro. Serum-free porcine myotube cultures expressing the estrogen receptors ERα and ERβ were treated with various concentrations of genistein, daidzein, or 17β-estradiol for 26 h. The degree of differentiation by creatine phosphokinase activity was not altered by treatment. At 100 μmol/L both genistein and daidzein caused decreases in protein amount due to cell loss. In addition, 100 μmol/L genistein reduced protein synthesis rate of the surviving cells (P < 0.05) measured as [3H]-phenylalanine incorporation. Interestingly, genistein (0.1 μmol/L), daidzein (10, 100 μmol/L), and 17β-estradiol (0.1, 1 nmol/L) slightly reduced protein degradation (P < 0.05). The results suggest that both genistein and daidzein affect protein metabolism in a dose-dependent manner and that estrogenic actions may play a role in decreasing protein degradation in porcine skeletal muscle.
Bibliography:http://dx.doi.org/10.1021/jf803039b
ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0021-8561
1520-5118
DOI:10.1021/jf803039b