Nutriomic Analysis of Fresh and Processed Fruit Products. 2. During in Vitro Simultaneous Molecular Passages Using Caco-2 Cell Monolayers

• Published in: Journal of agricultural and food chemistry Vol. 57; no. 8; pp. 3377 - 3388
• Main Authors: Epriliati, Indah, D’Arcy, Bruce, Gidley, Mike
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 22.04.2009
• Subjects: bioavailability; Biological and medical sciences; Caco-2 Cells; carboxylic acids; Carboxylic Acids - pharmacokinetics; Carica - chemistry; carotenoids; Carotenoids - pharmacokinetics; cell lines; Dietary Carbohydrates - pharmacokinetics; Digestion; dried fruit; dried vegetables; Food Handling; Food industries; Fruit - chemistry; Fruit and vegetable industries; fruit juices; Fundamental and applied biological sciences. Psychology; Humans; in vitro digestion; Intestinal Absorption; intestinal mucosa; Lycopersicon esculentum - chemistry; Mangifera - chemistry; mangoes; Models, Biological; Molecular Nutrition; nutrient availability; nutrient transport; nutrient uptake; nutrients; nutriomics; Nutritional Physiological Phenomena; Nutritive Value; papayas; pectins; Pectins - metabolism; phenolic compounds; raw fruit; raw vegetables; sugars; tomato juice; tomatoes; Nutriomic analysis; papaya; Caco-2 monolayers; in vitro passage; fresh and processed fruits; tomato; mango; Monolayer; Cell culture; Vegetables; Fruit; Papaya; Digestive system; Gut; Mango; Fruit product; Tomato; In vitro; Fresh product
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf802226n

Many studies have used Caco-2 cell monolayers as human intestinal absorption models. However, only a few studied digested foods, instead of pure standard compounds. Moreover, beneficial and nutritional molecules (nutriome) have not been investigated simultaneously. The present study explored nutriome passages from digest solution of fresh, dried, and juiced tomato, mango, and papaya using Caco-2 cell monolayers in apical→basolateral directions. A validation method using complementary TEER and P app values or internal standard caffeine is recommended because physiologically passive diffusion is unlikely to happen. Sugars were transported into basolateral sides, resulting in potential glucose equivalent bioavailability of 2.26−75 mg h−1/100 g (WB). Using sugar passage rates (DB) of juices as 100% references, the rate order was tomato (49.8% dried; 89.5% fresh) > mango (56.8% dried; 22.8% fresh) > papaya (18.7% dried; 36.7% fresh). Major indications that phytochemical absorption does not occur in the small intestine were obtained from the bioassay condition selected. Apical organic acid levels decreased, which occasionally were transported into basolateral sides, whereas the disappearances of apical carotenoids and phenolics were not. Pectin substances were predicted to be responsible for the disappearances of bioactive compounds in those pectin-rich fruits. Further investigations on the role of pectin substances in intestinal passages are recommended.