Photothermal Ablation of in Situ Renal Tumor by PEG-IR780-C13 Micelles and Near-Infrared Irradiation

• Published in: Molecular pharmaceutics Vol. 13; no. 3; pp. 829 - 838
• Main Authors: Qiu, Xuefeng, Xu, Linfeng, Zhang, Yanting, Yuan, Ahu, Wang, Kaikai, Zhao, Xiaozhi, Wu, Jinhui, Guo, Hongqian, Hu, Yiqiao
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 07.03.2016
• Subjects: Animals; Apoptosis - drug effects; Apoptosis - radiation effects; Cell Proliferation - drug effects; Cell Proliferation - radiation effects; Combined Modality Therapy; Flow Cytometry; Hyperthermia, Induced; Immunoenzyme Techniques; Indoles - pharmacology; Infrared Rays; Kidney Neoplasms - pathology; Kidney Neoplasms - therapy; Male; Mice; Mice, Inbred BALB C; Micelles; Microscopy, Confocal; Phototherapy; Polyethylene Glycols - chemistry; Spectroscopy, Near-Infrared; Tumor Cells, Cultured; thermal ablation; IR780; renal tumor; photothermal therapy
• ISSN: 1543-8384; 1543-8392
• DOI: 10.1021/acs.molpharmaceut.5b00734

PEG-IR780-C13 micelles have been demonstrated to be a novel photothermal agent with tumor-targeting property. This study was designed to explore the feasibility of applying PEG-IR780-C13 micelles and near-infrared (NIR) irradiation for thermal ablation of renal tumor by using an in situ tumor model. In addition, the potential thermal injury to normal renal tissue was evaluated. PEG-IR780-C13 micelles were intended to accumulate in renal tumor after systemic delivery. In vitro results revealed that PEG-IR780-C13 micelles were uptaken by RENCA cells mainly through caveola-mediated endocytosis and mainly distributed in late endosomes and lysosomes. Upon NIR irradiation, PEG-IR780-C13 micelles generated heat effectively both in vitro and in vivo, exhibiting a promising photothermal therapeutic property. The photothermal effect of PEG-IR780-C13 micelles could effectively destroy RENCA cells in vitro and adequately inhibit growth of in situ renal tumor in vivo. Meanwhile, PEG-IR780-C13 micelles mediated photothermal therapy (PTT) resulting in only limited injury to normal renal tissue surrounding tumor sites. Our data indicated that PEG-IR780-C13 micelles mediating PTT could generate tumor-specific heat for destruction of renal tumor in a minimally invasive way, providing a novel strategy for thermal ablation of renal tumor.