Quantitative Analysis of Caspase‑1 Activity in Living Cells Through Dynamic Equilibrium of Chlorophyll-Based Nano-assembly Modulated Photoacoustic Signals

In situ construction of self-assemblies with unique property in living systems is a promising direction in the biomedical field. The noninvasive methods for significant enzyme activity in living cells or living subjects are imperative and meantime challenge tasks. The dynamic process of self-assembl...

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Published inACS applied materials & interfaces Vol. 8; no. 28; pp. 17936 - 17943
Main Authors Li, Li-Li, Zeng, Qian, Liu, Wei-Jiao, Hu, Xue-Feng, Li, Yongsheng, Pan, Jie, Wan, Dong, Wang, Hao
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 20.07.2016
Subjects
Animals
bacterial infections
Caspase 1 - analysis
Caspase 1 - metabolism
caspase-1
chemical elements
Chlorophyll - chemistry
enzyme activity
Macrophages - enzymology
Mice
monitoring
Nanostructures - chemistry
Photoacoustic Techniques - methods
quantitative analysis
RAW 264.7 Cells
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Staphylococcal Infections - enzymology
Staphylococcus aureus
chlorophyll
self-assembly
dynamic equilibrium
bacterial infection
caspase-1
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Summary:In situ construction of self-assemblies with unique property in living systems is a promising direction in the biomedical field. The noninvasive methods for significant enzyme activity in living cells or living subjects are imperative and meantime challenge tasks. The dynamic process of self-assembly of chlorophyll-based molecules in complex biological systems can be monitored by photoacoustic signals, which supports a noninvasive way to understand and quantitatively measure the activity of caspase-1. Furthermore, the activity of caspase-1 enables reflection of the bacterial infection in the early stage. Here, we present a biocompatible self-assembly from chlorophyll-peptide derivatives and first correlate the dynamic equilibrium with ratiometric photoacoustic signals. The intracellular equilibrium was managed by a bacterial infection precaution protein, i.e., caspase-1. This system offers a trial of noninvasive method to quantitative detection and real-time monitoring of bacterial infection in the early stage.
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ISSN:1944-8244
1944-8252
1944-8252
DOI:10.1021/acsami.6b05795