Water-Soluble Supramolecular Polymers of Paired and Stacked Heterocycles: Assembly, Structure, Properties, and a Possible Path to Pre-RNA

• Published in: Journal of the American Chemical Society Vol. 143; no. 25; pp. 9279 - 9296
• Main Authors: Schuster, Gary B, Cafferty, Brian J, Karunakaran, Suneesh C, Hud, Nicholas V
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 30.06.2021
• Subjects: Evolution, Chemical; Heterocyclic Compounds - chemistry; Hydrogen Bonding; Macromolecular Substances - chemistry; Molecular Conformation; Polymers - chemistry; RNA - chemistry; Solubility; Water - chemistry
• ISSN: 0002-7863; 1520-5126
• DOI: 10.1021/jacs.0c13081

The hypothesis that RNA and DNA are products of chemical and biological evolution has motivated our search for alternative nucleic acids that may have come earlier in the emergence of lifepolymers that possess a proclivity for covalent and non-covalent self-assembly not exhibited by RNA. Our investigations have revealed a small set of candidate ancestral nucleobases that self-assemble into hexameric rosettes that stack in water to form long, twisted, rigid supramolecular polymers. These structures exhibit properties that provide robust solutions to long-standing problems that have stymied the search for a prebiotic synthesis of nucleic acids. Moreover, their examination by experimental and computational methods provides insight into the chemical and physical principles that govern a particular class of water-soluble one-dimensional supramolecular polymers. In addition to efficient self-assembly, their lengths and polydispersity are modulated by a wide variety of positively charged, planar compounds; their assembly and disassembly are controlled over an exceedingly narrow pH range; they exhibit spontaneous breaking of symmetry; and homochirality emerges through non-covalent cross-linking during hydrogel formation. Some of these candidate ancestral nucleobases spontaneously form glycosidic bonds with ribose and other sugars, and, most significantly, functionalized forms of these heterocycles form supramolecular structures and covalent polymers under plausibly prebiotic conditions. This Perspective recounts a journey of discovery that continues to reveal attractive answers to questions concerning the origins of life and to uncover the principles that control the structure and properties of water-soluble supramolecular polymers.