Global resolution of heterogeneous decay by phase/modulation fluorometry: mixtures and proteins

Fluorescence lifetime data obtained by the phase/modulation technique are used to resolve the heterogeneous fluorescence decays of model mixtures and of horse liver alcohol dehydrogenase. This was made possible by a global nonlinear least-squares procedure for simultaneous analysis of multiple data...

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Bibliographic Details
Published inBiochemistry (Easton) Vol. 22; no. 26; pp. 6054 - 6058
Main Authors Beechem, Joseph M, Knutson, Jay R, Ross, J. B. Alexander, Turner, Benjamin W, Brand, Ludwig
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 01.12.1983
Subjects
alcohol dehydrogenase
Biological and medical sciences
fluorescence
Fundamental and applied biological sciences. Psychology
horses
liver
Molecular biophysics
Spectroscopy : techniques and spectras
Proteins
Lifetime
Fluorescent tracer
Phase modulation
Enzyme
Theory
Fluorescence
Alcohol dehydrogenase
Luminescence decay
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Summary:Fluorescence lifetime data obtained by the phase/modulation technique are used to resolve the heterogeneous fluorescence decays of model mixtures and of horse liver alcohol dehydrogenase. This was made possible by a global nonlinear least-squares procedure for simultaneous analysis of multiple data sets. Analyses of simulated data suggest that biexponential lifetimes that differ by as little as 20% may be resolvable by this method.
Bibliography:ark:/67375/TPS-3JVCGHLC-4
istex:87828B98F2C9727F33ADF94C419769F207F97041
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0006-2960
1520-4995
DOI:10.1021/bi00295a002