Connecting the Pipes: Agricultural Tile Drains and Elevated Imidacloprid Brain Concentrations in Juvenile Northern Leopard Frogs (Rana pipiens)

• Published in: Environmental science & technology Vol. 57; no. 7; pp. 2758 - 2767
• Main Authors: Campbell, Kaitlyn S., Keller, Peyton, Golovko, Svetlana A., Seeger, Drew, Golovko, Mikhail Y., Kerby, Jacob L.
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 21.02.2023
• Subjects: Amphibians; Animals; Blood-brain barrier; Brain; Cerebellum; Contaminants in Aquatic and Terrestrial Environments; Drains; Ecological conditions; Frogs; Imidacloprid; Insecticides; Low concentrations; Metabolism; Metabolites; Neonicotinoids; Neurotoxicity; Nitro Compounds; Nontarget organisms; Pesticides; Rana pipiens; Reptiles & amphibians; Tile drains; Water; Water analysis; Water Pollutants, Chemical - analysis; Water sampling; Wetlands; subsurface drainage systems; neurology; cerebellum; neonicotinoids; brain size; amphibians
• ISSN: 0013-936X; 1520-5851
• DOI: 10.1021/acs.est.2c06527

Neonicotinoids are neurotoxic insecticides and are often released into nearby wetlands via subsurface tile drains and can negatively impact nontarget organisms, such as amphibians. Previous studies have indicated that imidacloprid, a commonly used neonicotinoid, can cross the amphibian blood–brain barrier under laboratory conditions; however, little is known about the impact of low concentrations in a field-based setting. Here, we report aqueous pesticide concentrations at wetland production areas that were either connected or not connected to agricultural tile drains, quantified imidacloprid and its break down products in juvenile amphibian brains and livers, and investigated the relationship between imidacloprid brain concentration and brain size. Imidacloprid concentrations in brain and water samples were nearly 2.5 and 5 times higher at tile wetlands (brain = 4.12 ± 1.92 pg/mg protein; water = 0.032 ± 0.045 μg/L) compared to reference wetlands, respectively. Tile wetland amphibians also had shorter cerebellums (0.013 ± 0.001 mm), depicting a negative relationship between imidacloprid brain concentration and cerebellum length. The metabolite, desnitro-imidacloprid, had liver concentrations that were 2 times higher at tile wetlands (2 ± 0.3 μg/g). Our results demonstrate that imidacloprid can cross the amphibian blood–brain barrier under ecological conditions and may alter brain dimensions and provide insight into the metabolism of imidacloprid in amphibians.