High Performance Liquid Chromatography Method for Residues Analysis of Thidiazuron in Apple and Soil

• Published in: Bulletin of environmental contamination and toxicology Vol. 87; no. 4; pp. 448 - 451
• Main Authors: Hu, Ji-Ye, Hu, Ya-Qin, Chen, Yang, Yang, Tao
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.10.2011; Springer Nature B.V
• Subjects: Apples; Aquatic Pollution; Chromatography; Chromatography, Liquid - methods; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Fruits; Ionization; Liquid chromatography; Malus - chemistry; Mass spectrometry; Nitrogen; Pesticides; Phenylurea Compounds - analysis; Phenylurea Compounds - chemistry; Plant Growth Regulators - analysis; Plant Growth Regulators - chemistry; Pollution; Scientific imaging; Sensors; Soil - chemistry; Soil Pollutants - analysis; Soil Pollutants - chemistry; Soil Science & Conservation; Soils; Solid Phase Extraction; Standard deviation; Thiadiazoles - analysis; Thiadiazoles - chemistry; Waste Water Technology; Water Management; Water Pollution Control; Beijing China; China; Residues determination; High performance liquid chromatography with UV detector; Thidiazuron; Solid-phase extraction
• ISSN: 0007-4861; 1432-0800
• DOI: 10.1007/s00128-011-0383-z

A rapid, sensitive and reliable analytical method for thidiazuron residues in apple and soil was established. The residual levels of the pesticide in apple and soil were determined by high performance liquid chromatography (HPLC) with UV detector. Samples of apple and soil were extracted with acetonitrile–water solutions, and then cleaned up by Florisil or C 18 cartridges. The results showed good linearity ( r 2  = 1.000) over the concentration range of 0.01–5.0 mg/L. Limits of quantification (LOQ) of the method were 0.01 mg/kg for both soil and apple. Recovery from the apple and soil samples were 83.36%–84.08% and 85.27%–89.83%, respectively, and the corresponding relative standard deviations (RSDs) of the recovery data were 0.155%–0.524% and 0.475%–4.79% for the three fortified levels (0.01, 0.1, 0.5 mg/kg). The analyte in the samples were further confirmed by electrospray ionization tandem mass spectrometry (ESI–MS/MS). It was demonstrated that the proposed method was simple and efficient, and particularly suitable for detecting thidiazuron residues in apple and soil.