EPSPS Gene Amplification in Glyphosate-Resistant Italian Ryegrass (Lolium perenne ssp. multiflorum) Populations from Arkansas (United States)

• Published in: Journal of agricultural and food chemistry Vol. 63; no. 25; pp. 5885 - 5893
• Main Authors: Salas, Reiofeli A, Scott, Robert C, Dayan, Franck E, Burgos, Nilda R
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01.07.2015; American Chemical Society, Books and Journals Division
• Subjects: 3-phosphoshikimate 1-carboxyvinyltransferase; 3-Phosphoshikimate 1-Carboxyvinyltransferase - genetics; absorption; Arkansas; bioassays; dose response; Gene Amplification; Gene Dosage; genes; Glycine - analogs & derivatives; Glycine - pharmacology; Glyphosate; growth retardation; Herbicide Resistance; Herbicides - pharmacology; Lolium - drug effects; Lolium - genetics; Lolium - metabolism; Lolium multiflorum; Mutation; nucleotide sequences; Plant Proteins - genetics; Plant Proteins - metabolism; resistance mechanisms; sequence analysis; Arkansas; Italian ryegrass (Lolium perenne ssp. multiflorum); 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS); glyphosate resistance; gene amplification
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.5b00018

Glyphosate-resistant Italian ryegrass was detected in Arkansas (United States) in 2007. In 2014, 45 populations were confirmed resistant in eight counties across the state. The level of resistance and resistance mechanisms in six populations were studied to assess the severity of the problem and identify alternative management approaches. Dose–response bioassays, glyphosate absorption and translocation experiments, herbicide target (EPSPS) gene sequence analysis, and gene amplification assays were conducted. The dose causing 50% growth reduction (GR50) was 7–19 times higher for the resistant population than for the susceptible standard. Uptake and translocation of 14C-glyphosate were similar in resistant and susceptible plants, and no mutation in the EPSPS gene known to be associated with resistance to glyphosate was detected. Resistant plants contained from 11- to >100-fold more copies of the EPSPS gene than the susceptible plants, whereas the susceptible plants had only one copy of EPSPS. Plants surviving the recommended dose of glyphosate contained at least 10 copies. The EPSPS copy number was positively related to glyphosate resistance level (r = 80). Therefore, resistance to glyphosate in these populations is due to multiplication of the target site. Resistance mechanisms could be location-specific. Suppressing the mechanism for gene amplification may overcome resistance.