Targeted chemical nucleases

The design of functional molecules has been an active area of research in chemistry and molecular biology during the past decade, encompassing topics such as host-guest chemistry, ribozymes, catalytic antibodies, and most recently, fullerenes. Nucleolytic agents that cleave the phosphodiester backbo...

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Published in	Accounts of chemical research Vol. 26; no. 3; pp. 98 - 104
Main Authors	Sigman, David S, Bruice, Thomas W, Mazumder, Abhijit, Sutton, Christopher L
Format	Journal Article
Language	English
Published	United States American Chemical Society 01.03.1993
Subjects	550200 - Biochemistry AMINO ACIDS ANTIBODIES AROMATICS AZAARENES BASIC BIOLOGICAL SCIENCES CARBOXYLIC ACIDS CATALYSIS CHELATES CHELATING AGENTS CHEMICAL REACTIONS CLEAVAGE COMPLEXES COPPER COMPLEXES CRYSTAL STRUCTURE DECOMPOSITION DESIGN DNA EDTA ENZYMES ESTERASES GUANOSINE HETEROCYCLIC COMPOUNDS HYDROLASES HYDROLYSIS LYSIS MAPPING MICROSTRUCTURE MOLECULAR BIOLOGY NICKEL COMPLEXES NUCLEASES NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC ACIDS ORGANIC COMPOUNDS ORGANIC NITROGEN COMPOUNDS OXIDATION PEPTIDES PHOSPHODIESTERASES PROTEINS PURINES RHODIUM COMPLEXES RIBOSIDES RNA RUTHENIUM COMPLEXES SOLVOLYSIS SUBSTRATES SYNTHESIS TRANSITION ELEMENT COMPLEXES
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Summary:	The design of functional molecules has been an active area of research in chemistry and molecular biology during the past decade, encompassing topics such as host-guest chemistry, ribozymes, catalytic antibodies, and most recently, fullerenes. Nucleolytic agents that cleave the phosphodiester backbone of DNA and RNA have been another important focus of activity. Interest in this subject has paralleled, and relied on, rapid advances in molecular biology, genome mapping and sequencing, and also the chemical synthesis of deoxyoligonucleotides and peptides. The goals of this research, the methodological approaches, and the insights into chemical biology provided by these molecules are the topic of this Account, which will emphasize research with the chemical nuclease activity of 1,10-phenanthroline-copper by our laboratory and others but will also summarize the innovative studies of Dervan and colleagues using ferrous-EDTA linked to targeting molecules. (DBO)
Bibliography:	ark:/67375/TPS-K2CHG572-S istex:6967C2D3EBFCE303DF46CD81E12A3194562B63A4 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0001-4842 1520-4898
DOI:	10.1021/ar00027a004