Terbium(III) Luminescent Complexes as Millisecond-Scale Viscosity Probes for Lifetime Imaging

Fluorescent probes that are able to directly measure viscosity are attractive candidates for the study of intracellular environments. We report a new class of luminescent rotors, based on the sensitized emission of a terbium­(III) complex. A 4-fold increase in both quantum yield and luminescence lif...

Full description

Saved in:
Bibliographic Details
Published inJournal of the American Chemical Society Vol. 139; no. 23; pp. 7693 - 7696
Main Authors Bui, Anh Thy, Grichine, Alexei, Duperray, Alain, Lidon, Pierre, Riobé, François, Andraud, Chantal, Maury, Olivier
Format Journal Article
LanguageEnglish
Published WASHINGTON American Chemical Society 14.06.2017
Amer Chemical Soc
Subjects
Catalysis
Chemical Sciences
Chemistry
Chemistry, Multidisciplinary
Inorganic chemistry
Material chemistry
Organic chemistry
Physical Sciences
Science & Technology
ROTOR
LANTHANIDE COMPLEXES
BIOPROBES
SYSTEMS
SENSOR
MICROSCOPY
YTTERBIUM
LIVING CELLS
Online AccessGet full text

Cover

More Information
Summary:Fluorescent probes that are able to directly measure viscosity are attractive candidates for the study of intracellular environments. We report a new class of luminescent rotors, based on the sensitized emission of a terbium­(III) complex. A 4-fold increase in both quantum yield and luminescence lifetime was observed in viscous media for the studied complexes, with a lifetime ranging from 0.23 to 0.89 ms over a broad range of viscosities (0.6–1200 cP). The presented approach, relying on the millisecond-scale luminescence lifetime of the lanthanide ions, was applied to fixed T24 cancer cells using temporal sampling lifetime imaging microscopy.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0002-7863
1520-5126
DOI:10.1021/jacs.7b02951