High-resolution gas chromatographic high-resolution mass spectrometric analysis of human adipose tissue for 2,3,7,8-tetrachlorodibenzo-p-dioxin
An analytical method is described for measuring 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and other TCDD isomers in human tissue samples using a highly specific cleanup procedure and high-resolution gas chromatography/high-resolution mass spectrometry. The 2,3,7,8-TCDD is quantified by the isotope...
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Published in | Analytical chemistry (Washington) Vol. 58; no. 4; pp. 705 - 713 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Washington, DC
American Chemical Society
01.04.1986
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Subjects | |
Online Access | Get full text |
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Summary: | An analytical method is described for measuring 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and other TCDD isomers in human tissue samples using a highly specific cleanup procedure and high-resolution gas chromatography/high-resolution mass spectrometry. The 2,3,7,8-TCDD is quantified by the isotope dilution technique with ( super(13)C sub(12))2,3,7,8-TCDD as the external standard. Other TCDD isomers are estimated by use of relative response factors. The 1 part-per-trillion (pptr) limit of detection is adequate for determining 2,3,7,8-TCDD concentrations present in human adipose tissue specimens. An analytical accuracy is demonstrated by the results obtained in analyzing several spiked samples. The method is shown to be unaffected by a number of potentially interfering compounds. A series of quality control material is used to verify system performance. For samples containing 1.6 pptr 2,3,7,8-TCDD, a coefficient of variation (CV) of 19% is observed. For samples at the 8.4 pptr level, a CV of 11% is observed. |
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Bibliography: | ark:/67375/TPS-HCDZT5ZR-4 istex:3C05C1A5A3A7287FF2C1ED6910637DF5A605EB45 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0003-2700 1520-6882 |
DOI: | 10.1021/ac00295a010 |