High-resolution gas chromatographic high-resolution mass spectrometric analysis of human adipose tissue for 2,3,7,8-tetrachlorodibenzo-p-dioxin

An analytical method is described for measuring 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and other TCDD isomers in human tissue samples using a highly specific cleanup procedure and high-resolution gas chromatography/high-resolution mass spectrometry. The 2,3,7,8-TCDD is quantified by the isotope...

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Published inAnalytical chemistry (Washington) Vol. 58; no. 4; pp. 705 - 713
Main Authors Patterson, Donald G, Holler, James S, Lapeza, Chester R, Alexander, Louis R, Groce, Donald F, O'Connor, Ralph C, Smith, S. Jay, Liddle, John A, Needham, Larry L
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 01.04.1986
Subjects
Adipose Tissue - analysis
Applied sciences
Biological and medical sciences
Chemical and industrial products toxicology. Toxic occupational diseases
Dioxins - analysis
Exact sciences and technology
Gas Chromatography-Mass Spectrometry
Humans
Medical sciences
Other techniques and industries
Polychlorinated Dibenzodioxins - analysis
Toxicology
Various organic compounds
Assay
Human
Gas chromatography
Adipose tissue
Mass spectrometry
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Summary:An analytical method is described for measuring 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and other TCDD isomers in human tissue samples using a highly specific cleanup procedure and high-resolution gas chromatography/high-resolution mass spectrometry. The 2,3,7,8-TCDD is quantified by the isotope dilution technique with ( super(13)C sub(12))2,3,7,8-TCDD as the external standard. Other TCDD isomers are estimated by use of relative response factors. The 1 part-per-trillion (pptr) limit of detection is adequate for determining 2,3,7,8-TCDD concentrations present in human adipose tissue specimens. An analytical accuracy is demonstrated by the results obtained in analyzing several spiked samples. The method is shown to be unaffected by a number of potentially interfering compounds. A series of quality control material is used to verify system performance. For samples containing 1.6 pptr 2,3,7,8-TCDD, a coefficient of variation (CV) of 19% is observed. For samples at the 8.4 pptr level, a CV of 11% is observed.
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ISSN:0003-2700
1520-6882
DOI:10.1021/ac00295a010