Cell-Permeant Large Stokes Shift Dyes for Transfection-Free Multicolor Nanoscopy

We designed cell-permeant red-emitting fluorescent dye labels with >140 nm Stokes shifts based on 9-imino­anthrone, 9-imino-10-sila­xanthone, and 9-imino-10-germa­xanthone fluoro­phores. The corresponding probes selectively targeting mito­chondria, lyso­somes, and F-actin demonstrate low toxicity...

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Bibliographic Details
Published inJournal of the American Chemical Society Vol. 139; no. 36; pp. 12378 - 12381
Main Authors Butkevich, Alexey N, Lukinavičius, Gražvydas, D’Este, Elisa, Hell, Stefan W
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 13.09.2017
Subjects
actin
fibroblasts
fluorescent dyes
human cell lines
humans
lysosomes
mitochondria
neurons
toxicity
wavelengths
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Summary:We designed cell-permeant red-emitting fluorescent dye labels with >140 nm Stokes shifts based on 9-imino­anthrone, 9-imino-10-sila­xanthone, and 9-imino-10-germa­xanthone fluoro­phores. The corresponding probes selectively targeting mito­chondria, lyso­somes, and F-actin demonstrate low toxicity and enable stimulated emission depletion (STED) nanoscopy in neurons, human fibroblasts, U2OS, and HeLa cells. In combination with known small Stokes shift dyes, our probes allow live-cell three-color STED nanoscopy of endogenous targets on popular setups with 775 nm STED wavelength.
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ISSN:0002-7863
1520-5126
1520-5126
DOI:10.1021/jacs.7b06412