Long-Term Detection of Oncogenic MicroRNA in Living Human Cancer Cells by Gold@ Polydopamine–Shell Nanoprobe

Oncogenic microRNAs (miRNA), for example, miR-155, are key tumor biomarkers in cancer cells that drive tumorigenesis, and the miRNA profile signature can predict cancer development and aggressiveness. Hence, timely detection of oncogenic miRNA in living cells is highly attractive to the diagnosis of...

Full description

Saved in:
Bibliographic Details
Published inACS biomaterials science & engineering Vol. 6; no. 7; pp. 3778 - 3783
Main Authors WONG, Wai Ki, Wong, Siu Hong Dexter, Bian, Liming
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 13.07.2020
Subjects
Carcinogenesis
Gold
Humans
Indoles
MicroRNAs - genetics
Polymers
cancer cellular uptake
gold nanoprobes
long-term real-time imaging
fluorescence quenching
oncogenic miRNA
Online AccessGet full text

Cover

More Information
Summary:Oncogenic microRNAs (miRNA), for example, miR-155, are key tumor biomarkers in cancer cells that drive tumorigenesis, and the miRNA profile signature can predict cancer development and aggressiveness. Hence, timely detection of oncogenic miRNA in living cells is highly attractive to the diagnosis of cancer at an early stage. Herein, we report a highly sequence-specific gold@polydopamine-based nanoprobe for long-term detection of miRNA in human cancer cell lines in vitro. A single administration of the nanoprobe enables continuous detection of the miR-155 expression level in living cancer cells for up to 5 days. We believe that our nanoprobe is highly promising for both oncology research and translational applications.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2373-9878
2373-9878
DOI:10.1021/acsbiomaterials.0c00633