5‑Formylcytosine Yields DNA–Protein Cross-Links in Nucleosome Core Particles

• Published in: Journal of the American Chemical Society Vol. 139; no. 31; pp. 10617 - 10620
• Main Authors: Li, Fengchao, Zhang, Yingqian, Bai, Jing, Greenberg, Marc M, Xi, Zhen, Zhou, Chuanzheng
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 09.08.2017; Amer Chemical Soc
• Subjects: Chemistry; Chemistry, Multidisciplinary; crosslinking; Cytosine - analogs & derivatives; Cytosine - chemistry; DNA; DNA - chemistry; DNA - metabolism; Electrophoresis, Polyacrylamide Gel; histones; Kinetics; Models, Biological; Molecular Structure; nucleosomes; Nucleosomes - chemistry; Physical Sciences; primary amines; Proteins - chemistry; Proteins - metabolism; Schiff Bases - chemistry; Science & Technology; TET ENZYMES; OXIDATION; ABASIC SITES; 5-METHYLCYTOSINE; RNA; BASE-RESOLUTION; HISTONE-CATALYZED CLEAVAGE; TRANSCRIPTION; 5-HYDROXYMETHYL; 5-CARBOXYLCYTOSINE

In situ generation of 5-formylcytosine (5fC) in nucleosome core particles (NCPs) reveals that 5fC leads to essential DNA–protein cross-links (DPCs). Mechanistic studies using chemical models and mutated histones demonstrate that DPCs form reversibly between the formyl function of 5fC and primary ami...