5‑Formylcytosine Yields DNA–Protein Cross-Links in Nucleosome Core Particles

In situ generation of 5-formylcytosine (5fC) in nucleosome core particles (NCPs) reveals that 5fC leads to essential DNA–protein cross-links (DPCs). Mechanistic studies using chemical models and mutated histones demonstrate that DPCs form reversibly between the formyl function of 5fC and primary ami...

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Published inJournal of the American Chemical Society Vol. 139; no. 31; pp. 10617 - 10620
Main Authors Li, Fengchao, Zhang, Yingqian, Bai, Jing, Greenberg, Marc M, Xi, Zhen, Zhou, Chuanzheng
Format Journal Article
LanguageEnglish
Published WASHINGTON American Chemical Society 09.08.2017
Amer Chemical Soc
Subjects
Chemistry
Chemistry, Multidisciplinary
crosslinking
Cytosine - analogs & derivatives
Cytosine - chemistry
DNA
DNA - chemistry
DNA - metabolism
Electrophoresis, Polyacrylamide Gel
histones
Kinetics
Models, Biological
Molecular Structure
nucleosomes
Nucleosomes - chemistry
Physical Sciences
primary amines
Proteins - chemistry
Proteins - metabolism
Schiff Bases - chemistry
Science & Technology
TET ENZYMES
OXIDATION
ABASIC SITES
5-METHYLCYTOSINE
RNA
BASE-RESOLUTION
HISTONE-CATALYZED CLEAVAGE
TRANSCRIPTION
5-HYDROXYMETHYL
5-CARBOXYLCYTOSINE
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Summary:In situ generation of 5-formylcytosine (5fC) in nucleosome core particles (NCPs) reveals that 5fC leads to essential DNA–protein cross-links (DPCs). Mechanistic studies using chemical models and mutated histones demonstrate that DPCs form reversibly between the formyl function of 5fC and primary amines on histones. These results suggest that DPC formation from 5fC in chromatin occurs in addition to its role in DNA demethylation.
Bibliography:NIH RePORTER
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ISSN:0002-7863
1520-5126
1520-5126
DOI:10.1021/jacs.7b05495