Integration of Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes with Hydroxyapatite-Embedded Hyaluronic Acid-Alginate Hydrogel for Bone Regeneration

• Published in: ACS biomaterials science & engineering Vol. 6; no. 3; pp. 1590 - 1602
• Main Authors: Yang, Shuo, Zhu, Biao, Yin, Peng, Zhao, Lisheng, Wang, Yizhu, Fu, Zhiguang, Dang, Ruijie, Xu, Juan, Zhang, Jianjun, Wen, Ning
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 09.03.2020
• Subjects: Alginates; Animals; Bone Regeneration; Durapatite; Exosomes; Humans; Hyaluronic Acid; Hydrogels; Mesenchymal Stem Cells; Mice; Osteogenesis; Rats; Umbilical Cord; osteogenesis; hydrogel; human umbilical cord mesenchymal stem cells; exosomes
• ISSN: 2373-9878; 2373-9878
• DOI: 10.1021/acsbiomaterials.9b01363

The treatment of bone defects has plagued clinicians. Exosomes, the naturally secreted nanovesicles by cells, exhibit great potential in bone defect regeneration to realize cell-free therapy. In this work, we successfully revealed that human umbilical cord mesenchymal stem cells-derived exosomes could effectively promote the proliferation, migration, and osteogenic differentiation of a murine calvariae preosteoblast cell line in vitro. Considering the long period of bone regeneration, to effectively exert the reparative effect of exosomes, we synthesized an injectable hydroxyapatite (HAP)-embedded in situ cross-linked hyaluronic acid-alginate (HA-ALG) hydrogel system to durably retain exosomes at the defect sites. Then, we combined the exosomes with the HAP-embedded in situ cross-linked HA-ALG hydrogel system to repair bone defects in rats in vivo. The results showed that the combination of exosomes and composite hydrogel could significantly enhance bone regeneration. Our experiment provides a new strategy for exosome-based therapy, which shows great potential in future tissue and organ repair.