Hairpin and duplex forms of a self-complementary dodecamer, d-AGATCTAGATCT, and interaction of the duplex form with the peptide KGWGK: can a pentapeptide destabilize DNA?

• Published in: Biochemistry (Easton) Vol. 31; no. 27; pp. 6241 - 6245
• Main Authors: Roy, Kunal B, Kukreti, Shrikant, Bose, Himangshu S, Chauhan, V. S, Rajeswari, Moganty R
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 14.07.1992
• Subjects: Amino Acid Sequence; Base Sequence; Circular Dichroism; DNA - chemistry; Drug Stability; Intercalating Agents; Models, Molecular; Molecular Sequence Data; Nucleic Acid Conformation; Nucleic Acid Denaturation; Oligodeoxyribonucleotides - chemistry; Oligopeptides - chemistry; Protein Binding; Protein Conformation; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Thermodynamics
• ISSN: 0006-2960; 1520-4995
• DOI: 10.1021/bi00142a011

Ordered forms of a synthetic dodecamer, d-AGATCTAGATCT, a direct repeat of the BglII recognition sequence, have been investigated using UV, CD, and fluorescence spectroscopy. Complex hairpin-duplex equilibria are manifest in UV thermal transitions, which are monophasic in the presence of very low or high NaCl concentrations but distinctly biphasic at intermediate ionic strengths. In 100 mM NaCl, the 1/Tm vs 1n C curve has a reasonable positive slope, which yields delta H and delta S for duplex formation as -66.2 kcal/mol and -190 cal/mol, respectively. Interaction of the dodecamer in duplex form with a tryptophan-containing peptide, KGWGK, has also been investigated to test the "bookmark" hypothesis (Gabbay et al., 1976) under the uniform structural constraint of the oligonucleotide of defined sequence. CD spectra of the peptide (P), the oligonucleotide (N), and their mixtures at different P/N ratios show a dramatic change in peptide spectrum but little change in nucleic acid dichroism with peptide binding. The Tm of P-N complexes decreases with an increase in peptide binding and levels off at saturation binding of P/N = 2.0. The data are interpreted in terms of a groove-cum-intercalation mode of binding, where intercalation to the tryptophan side chain destabilizes the double helix. A Scatchard plot of the binding data is nonlinear, with best-fit values for an overall association constant K = 4.33 x 10(5) M-1, and the number of binding sites n = 3.23 when fitted to the site-exclusion model of binding.