Structural limitations on the bifunctional intercalation of diacridines into DNA

• Published in: Biochemistry (Easton) Vol. 17; no. 23; pp. 5057 - 5063
• Main Authors: Wakelin, L. P. G, Romanos, M, Chen, T. K, Glaubiger, D, Canellakis, E. S, Waring, M. J
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01.11.1978
• Subjects: Acridines; Animals; Cattle; Chemical Phenomena; Chemistry; DNA; DNA, Circular; Molecular Conformation; Nucleic Acid Conformation; Structure-Activity Relationship; Thymus Gland; Viscosity
• ISSN: 0006-2960; 1520-4995
• DOI: 10.1021/bi00616a031

An homologous series of diacridines containing two 9-aminoacridine chromophores linked via a simple methylene chain has been studied in order to investigate the minimum interchromophore separation required to permit bifunctional intercalation. Viscometric, sedimentation, and electric dichroism experiments show that compounds having one to four methylene groups in the linker are restricted to monofunctional intercalation, whereas the interaction becomes bifunctional when the chain length is increased to six carbons or more. The results indicate that bifunctional reaction occurs with an interchromophore distance not exceeding 8.8 A, implying that intercalation by these compounds is not subject to neighbor exclusion if the mode of binding is of the classical intercalation type.