Polysaccharides from the Cell Walls of Pineapple Fruit

• Published in: Journal of agricultural and food chemistry Vol. 43; no. 3; pp. 608 - 612
• Main Authors: Bartolome, Ana P, Ruperez, Pilar, Prieto, Alicia
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.03.1995
• Subjects: ANANAS (FRUITS); ARABINOSA; ARABINOSE; Biological and medical sciences; CELL WALLS; COMPOSITION; ESPECTROMETRIA; EXTRACCION; EXTRACTION; Food industries; Fruit and vegetable industries; Fundamental and applied biological sciences. Psychology; GALACTOSA; GALACTOSE; GLUCOSA; GLUCOSE; HEMICELLULOSE; HEMICELULOSA; MONOSACCHARIDE COMPOSITION; PARED CELULAR; PAROI CELLULAIRE; PINA; PINEAPPLES; POLISACARIDOS; POLYHOLOSIDE; POLYSACCHARIDES; PURIFICACION; PURIFICATION; SPECTRAL DATA; SPECTROMETRIE; SPECTROMETRY; XILOSA; XYLOSE; Monocotyledones; Hemicellulose; Extraction process; Cell wall; Ion exchange chromatography; Gas chromatography; Tropical fruit; Angiospermae; Analytical method; Bromeliaceae; Spermatophyta; Pineapple; Chemical composition; Polysaccharide; Mass spectrometry
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf00051a010

Cell wall material from pineapple fruit was obtained and sequentially extracted with 50 mM NaOH at 2 degrees C, 1 M KOH at 2 and 20 degrees C, and 4 M KOH at 20 degrees C. From the 4 M KOH-soluble fraction, a "neutral" polysaccharide and an acidic one were isolated by chloride anion exchange chromatography. The "neutral" fraction was composed of xylose, arabinose, glucose, galactose, and minor quantities of mannose. The infrared spectra showed an absorption band at 900 cm-1 of beta-linked polysaccharides. The methylation analysis revealed the main glycosidic linkages of the "neutral" polysaccharide: 1,4-linked xylose with branching points in C3 and C2, 1,4-linked glucopyranose with branching points in C6, and terminal glucopyranosyl, arabinofuranosyl, and arabino/xylopyranosyl residues