A New Assay for Endonuclease/Methyltransferase Activities Based on Graphene Oxide

A new endonuclease/methyltransferase activity assay method based on graphene oxide (GO) is developed. Substrate DNA is designed to possess a double-stranded part to serve as a nuclease substrate and a single-stranded part for anchoring the DNA to the GO surface via strong noncovalent binding. Nuclea...

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Published inAnalytical chemistry (Washington) Vol. 83; no. 23; pp. 8906 - 8912
Main Authors Lee, Jieon, Kim, Young-Kwan, Min, Dal-Hee
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 01.12.2011
Subjects
Analytical chemistry
Chemistry
Deoxyribonucleic acid
DNA
DNA - metabolism
Endonucleases - metabolism
Exact sciences and technology
Fluorescence
Graphite - chemistry
Hydrolysis
Measurement
Methyltransferases - metabolism
Oxides - chemistry
Spectrometric and optical methods
Spectrometry, Fluorescence
Substrates
Chemical analysis
Dyes
Enzyme
Fluorescence
Esterases
Method
Real time
Fluorescence spectrometry
Design
Hydrolysis
Substrate
Double stranded DNA
Non covalent binding
Hydrolases
End
Nuclease
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Summary:A new endonuclease/methyltransferase activity assay method based on graphene oxide (GO) is developed. Substrate DNA is designed to possess a double-stranded part to serve as a nuclease substrate and a single-stranded part for anchoring the DNA to the GO surface via strong noncovalent binding. Nuclease-mediated DNA hydrolysis induces the recovery of fluorescence intensity of the dye attached to the end of the double-stranded DNA region. This GO-based method allows real-time measurement and quantitative assay for endonuclease/methyltransferase activities in short time.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0003-2700
1520-6882
DOI:10.1021/ac201298r