Electron Tomography Shows Molecular Anchoring Within a Layer-by-Layer Film

The layer-by-layer self-assembly of thin films consisting of alternating layers of DNA and bis-urea nanoribbons prevents diffusion of the components within the film and allows the anchoring of biotinylated molecules through molecular recognition in a predetermined layer of the film. Electron tomogra...

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Bibliographic Details
Published inJournal of the American Chemical Society Vol. 130; no. 38; pp. 12608 - 12609
Main Authors Vos, Matthijn R. J, Breurken, Monica, Leclère, Philippe E. L. G, Bomans, Paul H. H, de Haas, Felix, Frederik, Peter M, Jansen, John A, Nolte, Roeland J. M, Sommerdijk, Nico A. J. M
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 24.09.2008
Subjects
Biotin - chemistry
DNA - chemistry
Electron Microscope Tomography - methods
Nanostructures - chemistry
Streptavidin - chemistry
Surface-Active Agents - chemistry
Urea - chemistry
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Summary:The layer-by-layer self-assembly of thin films consisting of alternating layers of DNA and bis-urea nanoribbons prevents diffusion of the components within the film and allows the anchoring of biotinylated molecules through molecular recognition in a predetermined layer of the film. Electron tomography demonstrates with nanometer precision the location of gold-labeled streptavidin bound to the incorporated biotinylated molecules.
Bibliography:istex:8212D90CD6A4DFA0649CEFDD79FACDF466E7A8D7
Experimental details, spectroscopic data and movie of the tilt series, reconstruction and surface rendering of the 3D tomographic volume. This material is available free of charge via the Internet at http://pubs.acs.org.
ark:/67375/TPS-CX86MTDF-T
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SourceType-Scholarly Journals-1
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ISSN:0002-7863
1520-5126
DOI:10.1021/ja804930d