An NMR Experiment for Simultaneous TROSY-Based Detection of Amide and Methyl Groups in Large Proteins

A sensitive 2D NMR experiment for simultaneous time-shared TROSY-type detection of amide and methyl groups in high-molecular-weight proteins is described. The pulse scheme is designed to preserve the slowly decaying components of both 1H−15N and methyl 13CH3 spin systems in the course of indirect ev...

Full description

Saved in:
Bibliographic Details
Published inJournal of the American Chemical Society Vol. 130; no. 33; pp. 10872 - 10873
Main Authors Guo, Chenyun, Zhang, Daoning, Tugarinov, Vitali
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 20.08.2008
Subjects
Amides - analysis
Macromolecular Substances - analysis
Magnetic Resonance Spectroscopy - methods
Magnetic Resonance Spectroscopy - standards
Proteins - analysis
Reference Standards
Online AccessGet full text

Cover

More Information
Summary:A sensitive 2D NMR experiment for simultaneous time-shared TROSY-type detection of amide and methyl groups in high-molecular-weight proteins is described. The pulse scheme is designed to preserve the slowly decaying components of both 1H−15N and methyl 13CH3 spin systems in the course of indirect evolution and acquisition periods. The proposed methodology is applied to the study of substrate binding to {U-[15N,2H]; Ile-[13CH3]; Leu,Val-[13CH3/12CD3]}-labeled 82-kDa enzyme Malate Synthase G and is expected to accelerate NMR-based screening of large proteins labeled with 15N and selectively labeled with 13CH3 at methyl sites.
Bibliography:ark:/67375/TPS-WK2340GW-V
istex:0ECBBD8CE90075A8F3B6857F1FBBA1149AA19449
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0002-7863
1520-5126
DOI:10.1021/ja8036178