Improved determination of phytate by ion-exchange chromatography

• Published in: Journal of agricultural and food chemistry Vol. 39; no. 10; pp. 1770 - 1772
• Main Authors: Bos, Klaas D, Verbeek, Cornelis, Van Eeden, C. H. Peter, Slump, Pier, Wolters, Mechteldis G. E
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.10.1991
• Subjects: ACIDE PHYTIQUE; ACIDO FITICO; ALIMENTOS; Biological and medical sciences; CHROMATOGRAPHIE; CROMATOGRAFIA; ECHANGE D'ION; Food industries; Fundamental and applied biological sciences. Psychology; General aspects; INTERCAMBIO IONICO; Methods of analysis, processing and quality control, regulation, standards; PRODUIT ALIMENTAIRE; TECHNIQUE ANALYTIQUE; TECNICAS ANALITICAS; HPLC chromatography; EDTA; Phytic acid; Antinutrient factor; Analytical method; Ion exchange chromatography
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf00010a015

An improved method for the ion chromatographic determination of phytic acid is presented. The method is based on a recently published chromatographic method combined with a treatment of the samples with EDTA. Separation and quantitation of phytic acid were performed on an anion-exchange column. Phytic acid was detected at 300 nm after reaction with a ferric salt in an in-line postcolumn derivatization. The improved method described here was compared with a recently published HPLC method without EDTA treatment and with a method using small single-use ion-exchange columns. It was found that the HPLC method without EDTA treatment of the samples results in values too low for phytic acid, whereas the method using small single-use ion-exchange columns gives values too high. The differences between the results of the three methods were statistically significant (p 0.01)