On the Influence of Different Surfaces in Nano- and Submicrometer Particle Based Fluorescence Immunoassays

Recently, numerous attempts have been made to improve the performance of fluorescence immunoassays. One way pursued is the substitution of labeling molecules by micro- or nanocrystalline dyes. The surfaces of these particulate structures are typically engineered by a layerwise assembly of oppositely...

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Bibliographic Details
Published inLangmuir Vol. 20; no. 21; pp. 9371 - 9379
Main Authors Bruemmel, Yvonne, Chan, Cangel Pui-yee, Renneberg, Reinhard, Thuenemann, Andreas, Seydack, Matthias
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 12.10.2004
Subjects
Ethylene Glycols - chemistry
Fluorescence
Glycerol - analogs & derivatives
Glycerol - chemistry
Imines - chemistry
Immunoassay - methods
Molecular Structure
Nanostructures - chemistry
Particle Size
Polyethylenes - chemistry
Surface Properties
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Summary:Recently, numerous attempts have been made to improve the performance of fluorescence immunoassays. One way pursued is the substitution of labeling molecules by micro- or nanocrystalline dyes. The surfaces of these particulate structures are typically engineered by a layerwise assembly of oppositely charged polyelectrolytes, the outer layer being constituted of biorecognition molecules, for example, immunoglobulins. In this study, we show that amphiphilic polymers such as alkylated poly(ethylene imine)s and 1,2-distearoyl-sn-glycero-3-phosphatoethanolamine-N-[amino(poly(ethylene glycol))] can fully substitute the more intricate layer-by-layer technique and evaluate the influence of surface charge and particle size on the overall performance of these assays.
Bibliography:ark:/67375/TPS-DX8TM9F5-H
istex:A181510548CFA27975F0A718ADE151A5A6ED2C76
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0743-7463
1520-5827
DOI:10.1021/la0486032