A Caged, Localizable Rhodamine Derivative for Superresolution Microscopy

A caged rhodamine 110 derivative for the specific labeling of SNAP-tag fusion proteins is introduced. The caged rhodamine 110 derivative permits the labeling of cell surface proteins in living cells and of intracellular proteins in fixed cells. The probe requires only a single caging group to mainta...

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Published inACS chemical biology Vol. 7; no. 2; pp. 289 - 293
Main Authors Banala, Sambashiva, Maurel, Damien, Manley, Suliana, Johnsson, Kai
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 17.02.2012
Subjects
Cell Line, Tumor
Fluorescent Dyes - analysis
HEK293 Cells
Humans
Microscopy, Fluorescence
Proteins - analysis
Recombinant Fusion Proteins - analysis
Rhodamines - analysis
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Summary:A caged rhodamine 110 derivative for the specific labeling of SNAP-tag fusion proteins is introduced. The caged rhodamine 110 derivative permits the labeling of cell surface proteins in living cells and of intracellular proteins in fixed cells. The probe requires only a single caging group to maintain the fluorophore in a non-fluorescent state and becomes highly fluorescent after uncaging. The high contrast ratio is confirmed both in bulk and at the single molecule level. This property, together with its high photon yield makes it an excellent dye for photoactivated localization microscopy (PALM), as we demonstrate here.
Bibliography:SourceType-Other Sources-1
ObjectType-Article-2
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ObjectType-Correspondence-1
ISSN:1554-8929
1554-8937
DOI:10.1021/cb2002889