Fluorescent Proteins: Shine on, You Crazy Diamond

• Published in: Journal of the American Chemical Society Vol. 135; no. 7; pp. 2387 - 2402
• Main Authors: Dedecker, Peter, De Schryver, Frans C, Hofkens, Johan
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 20.02.2013
• Subjects: Biosensing Techniques; Crystallography, X-Ray; Diagnostic Imaging - trends; fluorescence; fluorescent proteins; image analysis; Luminescent Proteins - chemistry; Magnetic Resonance Spectroscopy; Models, Molecular; protein structure; spectral analysis; spectroscopy

In this Perspective we discuss recent trends in the development and applications of fluorescent proteins. We start by providing a historical and structural perspective of their spectroscopic and structural aspects and describe how these properties have made fluorescent proteins essential as ‘smart l...