Fluorescent Proteins: Shine on, You Crazy Diamond

In this Perspective we discuss recent trends in the development and applications of fluorescent proteins. We start by providing a historical and structural perspective of their spectroscopic and structural aspects and describe how these properties have made fluorescent proteins essential as ‘smart l...

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Bibliographic Details
Published inJournal of the American Chemical Society Vol. 135; no. 7; pp. 2387 - 2402
Main Authors Dedecker, Peter, De Schryver, Frans C, Hofkens, Johan
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 20.02.2013
Subjects
Biosensing Techniques
Crystallography, X-Ray
Diagnostic Imaging - trends
fluorescence
fluorescent proteins
image analysis
Luminescent Proteins - chemistry
Magnetic Resonance Spectroscopy
Models, Molecular
protein structure
spectral analysis
spectroscopy
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Summary:In this Perspective we discuss recent trends in the development and applications of fluorescent proteins. We start by providing a historical and structural perspective of their spectroscopic and structural aspects and describe how these properties have made fluorescent proteins essential as ‘smart labels’ for biosensing and advanced fluorescence imaging. We show that the strong link between the spectroscopic properties and protein structure and properties is a necessary element in these developments and that this dependence makes the proteins excellent model systems for a variety of fields. We pay particular attention to emerging or future research opportunities and unsolved questions.
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ISSN:0002-7863
1520-5126
1520-5126
DOI:10.1021/ja309768d