Janus Membranes Patch Achieves High-Quality Tendon Repair: Inhibiting Exogenous Healing and Promoting Endogenous Healing

• Published in: Nano letters Vol. 24; no. 14; pp. 4300 - 4309
• Main Authors: Xie, Xiaojing, Xu, Junjie, Ding, Danzhi, Lin, Jing, Han, Kang, Wang, Chaorong, Wang, Fujun, Zhao, Jinzhong, Wang, Lu
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 10.04.2024
• Subjects: exogenous healing; Janus membranes patch; tendon repair; tendon adhesion; endogenous healing
• ISSN: 1530-6984; 1530-6992
• DOI: 10.1021/acs.nanolett.4c00818

The imbalance between endogenous and exogenous healing is the fundamental reason for the poor tendon healing. In this study, a Janus patch was developed to promote endogenous healing and inhibit exogenous healing, leading to improved tendon repair. The upper layer of the patch is a poly­(dl-lactide-co-glycolide)/polycaprolactone (PLGA/PCL) nanomembrane (PMCP-NM) modified with poly­(2-methylacryloxyethyl phosphocholine) (PMPC), which created a lubricated and antifouling surface, preventing cell invasion and mechanical activation. The lower layer is a PLGA/PCL fiber membrane loaded with fibrin (Fb) (Fb-NM), serving as a temporary chemotactic scaffold to regulate the regenerative microenvironment. In vitro, the Janus patch effectively reduced 92.41% cell adhesion and 79.89% motion friction. In vivo, the patch inhibited tendon adhesion through the TGF-β/Smad signaling pathway and promoted tendon maturation. This Janus patch is expected to provide a practical basis and theoretical guidance for high-quality soft tissue repair.