Graphene-Based Chemiluminescence Resonance Energy Transfer for Homogeneous Immunoassay

We report on chemiluminescence resonance energy transfer (CRET) between graphene nanosheets and chemiluminescent donors. In contrast to fluorescence resonance energy transfer, CRET occurs via nonradiative dipole–dipole transfer of energy from a chemiluminescent donor to a suitable acceptor molecule...

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Bibliographic Details
Published inACS nano Vol. 6; no. 4; pp. 2978 - 2983
Main Authors Lee, Joon Seok, Joung, Hyou-Arm, Kim, Min-Gon, Park, Chan Beum
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 24.04.2012
Subjects
C-Reactive Protein - analysis
Chemiluminescence
Diseases
Energy Transfer
Fluorescence
Graphene
Graphite - chemistry
Hepatocytes - metabolism
Human
Humans
Immunoassay
Immunoassay - methods
Luminescent Measurements - methods
Models, Molecular
Molecular Conformation
Nanostructure
Platforms
CRET
immunosensors
C-reactive protein
chemiluminescence
graphene
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Summary:We report on chemiluminescence resonance energy transfer (CRET) between graphene nanosheets and chemiluminescent donors. In contrast to fluorescence resonance energy transfer, CRET occurs via nonradiative dipole–dipole transfer of energy from a chemiluminescent donor to a suitable acceptor molecule without an external excitation source. We designed a graphene-based CRET platform for homogeneous immunoassay of C-reactive protein (CRP), a key marker for human inflammation and cardiovascular diseases, using a luminol/hydrogen peroxide chemiluminescence (CL) reaction catalyzed by horseradish peroxidase. According to our results, anti-CRP antibody conjugated to graphene nanosheets enabled the capture of CRP at the concentration above 1.6 ngmL–1. In the CRET platform, graphene played a key role as an energy acceptor, which was more efficient than graphene oxide, while luminol served as a donor to graphene, triggering the CRET phenomenon between luminol and graphene. The graphene-based CRET platform was successfully applied to the detection of CRP in human serum samples in the range observed during acute inflammatory stress.
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ISSN:1936-0851
1936-086X
DOI:10.1021/nn300684d