Post-synthetically Ligated Ribozymes: An Alternative Approach to Iterative Solid-Phase Synthesis
To improve the overall yield of ribozyme synthesis, a convergent approach, based on the post-synthetic formation of an amino linker between two half-ribozymes was investigated. Borane·pyridine-mediated reductive amination of 3‘-phosphoglycaldehyde-5‘-half-ribozymes with 5‘-aminohexyl-3‘-half-ribozym...
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Published in | Bioconjugate chemistry Vol. 8; no. 2; pp. 204 - 212 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Chemical Society
01.03.1997
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Subjects | |
Online Access | Get full text |
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Summary: | To improve the overall yield of ribozyme synthesis, a convergent approach, based on the post-synthetic formation of an amino linker between two half-ribozymes was investigated. Borane·pyridine-mediated reductive amination of 3‘-phosphoglycaldehyde-5‘-half-ribozymes with 5‘-aminohexyl-3‘-half-ribozymes generated the corresponding amino-linked ribozymes in yields >77% on different scales. The investigation of a variety of reducing agents is discussed together with a kinetic analysis of the selected coupling reaction. These post-synthetically ligated ribozymes exhibited slightly reduced in vitro catalytic activity and cell efficacy. |
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Bibliography: | Abstract published in Advance ACS Abstracts, February 15, 1997. ark:/67375/TPS-SMB3C18X-L istex:B277A803261356026FE5656B70DE3EC78E1BDB4E ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1043-1802 1520-4812 |
DOI: | 10.1021/bc970011q |