Nanomechanical Signatures of Extracellular Vesicles from Hematologic Cancer Patients Unraveled by Atomic Force Microscopy for Liquid Biopsy

• Published in: Nano letters Vol. 23; no. 4; pp. 1591 - 1599
• Main Authors: Feng, Yaqi, Liu, Meichen, Li, Xinxin, Li, Mi, Xing, Xiaojing, Liu, Lianqing
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 22.02.2023
• Subjects: Extracellular Vesicles; Hematologic Neoplasms; Humans; Liquid Biopsy; Microscopy, Atomic Force - methods; Multiple Myeloma; hematologic cancer; atomic force microscopy; extracellular vesicle; nanomechanical properties; liquid biopsy
• ISSN: 1530-6984; 1530-6992
• DOI: 10.1021/acs.nanolett.3c00093

Cells release extracellular vesicles (EVs) as the carriers for intercellular communications to regulate life activities. Particularly, it is increasingly apparent that mechanical forces play an essential role in biological systems. The nanomechanical properties of EVs and their dynamics in cancer development are still not fully understood. Herein, with the use of atomic force microscopy (AFM), the nanomechanical signatures of EVs from the liquid biopsies of hematologic cancer patients were unraveled. Single native EVs were probed by AFM under aqueous conditions. The elastic and viscous properties of EVs were measured and visualized to correlate EV mechanics with EV geometry. Experimental results remarkably reveal the significant differences in EV mechanics among multiple myeloma patients, lymphoma patients, and healthy volunteers. The study unveils the unique nanomechanical signatures of EVs in hematologic cancers, which will benefit the studies of liquid biopsies for cancer diagnosis and prognosis with translational significance.