Bioluminescent Bacterial Genotoxicity Test for Fatty Acid Derivatives and Heated Oils

• Published in: Journal of agricultural and food chemistry Vol. 46; no. 7; pp. 2682 - 2685
• Main Authors: Sun, Thomas S. C, Stahr, Henry M, Love, Jane A
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.07.1998
• Subjects: ACEITE DE SOJA; ACEITES COMESTIBLES; ACIDE GRAS; ACIDOS GRASOS; ANALYTICAL METHODS; BACTERIA; Biological and medical sciences; BIOLUMINESCENCE; CALENTAMIENTO; CHAUFFAGE; Chemical mutagenesis; CHOLESTEROL; COLESTEROL; COMPOSE ORGANOCHLORE; COMPUESTO ORGANICO DEL CLORO; COOKING OILS; CORPS GRAS; ENSAYO; ESTER; ESTERES; ESTERS; FATS; FATTY ACIDS; Food industries; Food toxicology; Fundamental and applied biological sciences. Psychology; GENOTOXICIDAD; GENOTOXICITE; GENOTOXICITY; GRASAS; HEATING; HUILE DE CUISINE; HUILE DE SOJA; Medical sciences; ORGANOCHLORINE COMPOUNDS; OXIDACION; OXIDATION; OXIDIZED FATTY ACIDS; OXYDATION; RAPID METHODS; SOYBEAN OIL; TECHNIQUE ANALYTIQUE; TECNICAS ANALITICAS; TESTAGE; TESTING; Toxicology; Bioluminescence; Organic hydroperoxide; Mutagenicity testing; Lipids; Foodstuff; Edible oil; Microsome; Fatty acids; Salmonella typhimurium; Cholesterol; Chlorine Organic compounds; S9 mix; Metabolic activation; Bacteria; Oxidation; Degradation product; Enterobacteriaceae; Thermal degradation
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf970647b

The Mutatox test (the commercial name for a bioluminescent bacterial genotoxicity test), which has been proved to be a good alternative to the Ames test, was used in this study to test the genotoxicity of fatty acid derivatives and heated oils. This test allows either pure or complex compounds to be rapidly screened for genotoxicity. Five different column fractions isolated from autoxidized methyl linoleate or autoxidized methyl linolenate were not genotoxic with or without the activation by the S-9 (microsomal) fraction. Chlorinated fatty acids including chloroethyl caprate, laurate, palmitate, and linoleate were genotoxic in the presence of S-9 activation. When heated at 180 °C, after 32 h, soybean oil exhibited genotoxic activity with or without the S-9 activation. The addition of 1% cholesterol into the soybean oil increased the onset of the genotoxicity during heating. Keywords: Mutatox test; genotoxicity test; fatty acid hydroperoxides; chlorinated fatty acids