Hydrolyzed Caseinomacropeptide Conjugated Galactooligosaccharides Support the Growth and Enhance the Bile Tolerance in Lactobacillus Strains

• Published in: Journal of agricultural and food chemistry Vol. 60; no. 27; pp. 6839 - 6845
• Main Authors: Muthaiyan, Arunachalam, Hernandez-Hernandez, Oswaldo, Moreno, F. Javier, Sanz, Maria Luz, Ricke, Steven C
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 11.07.2012
• Subjects: Bile Acids and Salts - pharmacology; Biological and medical sciences; Caseins - chemistry; Caseins - metabolism; Fermentation; Food industries; Food microbiology; Fundamental and applied biological sciences. Psychology; Humans; Hydrolysis; Lactic Acid - metabolism; Lactobacillus - drug effects; Lactobacillus - growth & development; Lactobacillus - metabolism; Oligosaccharides - chemistry; Oligosaccharides - metabolism; Peptide Fragments - chemistry; Peptide Fragments - metabolism; Prebiotics - analysis; galactooligosaccharides; probiotic; prebiotic; Lactobacillus; Probiotic; Biological fluid; Lactic acid bacteria; Support; Growth; Tolerance; Caseinomacropeptide; Strain; Prebiotics; Bacteria; Lactobacillaceae; Bile
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf301392y

In this study bioactive caseinomacropeptide was conjugated with prebiotic galactooligosaccharides (hCMP:GOS) by Maillard reaction to synthesize value added prebiotic compounds to Lactobacillus strains. Growth study showed the ability of hCMP:GOS to serve as a sole carbon source for Lactobacillus strains. A significant amount of acetate and lactate was detected in cell free culture supernatant by HPLC. It demonstrated the ability of Lactobacillus strains to ferment the hCMP:GOS as a carbon source. In addition, hCMP:GOS grown Lactobacillus cells exhibited enhanced bile tolerance and retained 90% viability. Overall results of this study indicate that the hCMP conjugated GOS can be potential multipurpose prebiotic substrates to enhance the growth and bile tolerance in Lactobacillus strains and serve as a fermentable substrate to produce beneficial metabolites in the host.