Electrospinning of Bovine Serum Albumin. Optimization and the Use for Production of Biosensors

• Published in: Biomacromolecules Vol. 9; no. 7; pp. 2087 - 2090
• Main Authors: Kowalczyk, Tomasz, Nowicka, Aleksandra, Elbaum, Danek, Kowalewski, Tomasz A
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.07.2008
• Subjects: Animals; Applied sciences; Biological and medical sciences; Biosensing Techniques - methods; Biotechnology; Cattle; Exact sciences and technology; Fluorescein-5-isothiocyanate; Fundamental and applied biological sciences. Psychology; Machinery and processing; Methods. Procedures. Technologies; Nanostructures; Natural polymers; Physicochemistry of polymers; Plastics; Polymer industry, paints, wood; Protein Conformation; Proteins; Serum Albumin, Bovine - chemistry; Spinning; Technology of polymers; Various methods and equipments; Fluorescent tracer; Polymer blends; Labelled polymer; Electrospinning; Fluorescence; Nanofiber; Serum albumin; Experimental study; Protein; Ethylene oxide polymer; pH metering; Biosensor; Manufacturing; Intracellular; Spinnability
• ISSN: 1525-7797; 1526-4602
• DOI: 10.1021/bm800421s

Electrospinning of the globular protein, bovine serum albumin (BSA), was optimized to obtain proteinous fibers suitable as biosensors. It was shown that the as-spun protein preserves its native form, whereas solubility of the cross-linked in the ambient conditions BSA nanofibers evidently decreases. Insoluble BSA fibers can be easily modified to be used as two-dimensional biosensors. Here, we show the micro pH sensor obtained from the BSA fiber stained with a fluorescein derivative (FITC).