Electrophile Scanning Reveals Reactivity Hotspots for the Design of Covalent Peptide Binders

• Published in: ACS chemical biology Vol. 19; no. 1; pp. 101 - 109
• Main Authors: Grob, Nathalie M., Remarcik, Clint, Rössler, Simon L., Wong, Jeffrey Y. K., Wang, John C. K., Tao, Jason, Smith, Corey L., Loas, Andrei, Buchwald, Stephen L., Eaton, Dan L., López, Magdalena Preciado, Pentelute, Bradley L.
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 19.01.2024
• Subjects: Histocompatibility Antigens Class I - metabolism; HLA-E Antigens; Ligands; Peptides - chemistry; Protein Binding
• ISSN: 1554-8929; 1554-8937; 1554-8937
• DOI: 10.1021/acschembio.3c00538

Protein–protein interactions (PPIs) are intriguing targets in drug discovery and development. Peptides are well suited to target PPIs, which typically present with large surface areas lacking distinct features and deep binding pockets. To improve binding interactions with these topologies and advance the development of PPI-focused therapeutics, potential ligands can be equipped with electrophilic groups to enable binding through covalent mechanisms of action. We report a strategy termed electrophile scanning to identify reactivity hotspots in a known peptide ligand and demonstrate its application in a model PPI. Cysteine mutants of a known ligand are used to install protein-reactive modifiers via a palladium oxidative addition complex (Pd-OAC). Reactivity hotspots are revealed by cross-linking reactions with the target protein under physiological conditions. In a model PPI with the 9-mer peptide antigen VL9 and major histocompatibility complex (MHC) class I protein HLA-E, we identify two reactivity hotspots that afford up to 87% conversion to the protein–peptide conjugate within 4 h. The reactions are specific to the target protein in vitro and dependent on the peptide sequence. Moreover, the cross-linked peptide successfully inhibits molecular recognition of HLA-E by CD94–NKG2A possibly due to structural changes enacted at the PPI interface. The results illustrate the potential application of electrophile scanning as a tool for rapid discovery and development of covalent peptide binders.