GC/MS Determination of Pyrogallol and Gallic Acid in Biological Matrices as Diagnostic Indicators of Oak Exposure

A selective and sensitive method was developed for the quantitative determination of pyrogallol and detection of gallic acid in biological samples. Pyrogallol was measured as a metabolite of gallotannin in bovine urine and serum samples. Gallic acid was formed by hydrolysis of the gallotannins in bl...

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Bibliographic Details
Published inJournal of agricultural and food chemistry Vol. 44; no. 5; pp. 1275 - 1279
Main Authors Tor, Elizabeth R, Francis, Traci M, Holstege, Dirk M, Galey, Francis D
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 16.05.1996
Subjects
ACIDE ORGANIQUE
ACIDOS ORGANICOS
ANALISIS CUANTITATIVO
ANALYSE QUANTITATIVE
Biological and medical sciences
BOVIN
CHROMATOGRAPHIE EN PHASE GAZEUSE
COMPOSE AROMATIQUE
COMPOSE PHENOLIQUE
COMPUESTOS AROMATICOS
COMPUESTOS FENOLICOS
CROMATOGRAFIA DE GASES
ENVENENAMIENTO
ESPECTROMETRIA DE MASAS
Food industries
Food toxicology
Fundamental and applied biological sciences. Psychology
GANADO BOVINO
INTOXICATION
Medical sciences
ORINA
Plant poisons toxicology
QUERCUS
RUMEN
SERUM SANGUIN
SPECTROMETRIE DE MASSE
SUERO SANGUINEO
TANINOS
TANNIN
Toxicology
URINE
Gallic acid
Animal feeding
Coupled method
Biological marker
Plant leaf
Fagaceae
Pyrogallol
Quercus douglasii
Gas chromatography
Dicotyledones
Food poisoning
Angiospermae
Biological medium
Analytical method
Spermatophyta
Mass spectrometry
Quantitative analysis
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Summary:A selective and sensitive method was developed for the quantitative determination of pyrogallol and detection of gallic acid in biological samples. Pyrogallol was measured as a metabolite of gallotannin in bovine urine and serum samples. Gallic acid was formed by hydrolysis of the gallotannins in blue oak (Quercus douglasii) leaves, tannic acid standards, and rumen contents. Acid hydrolyzed or acidified samples were extracted with 5% ethanol in ethyl acetate (v/v), followed by evaporation and derivatization with Deriva-sil. The trimethylsilyl derivatives were analyzed by gas chromatography/mass spectrometry using selected ion monitoring. Method detection limits for gallic acid and pyrogallol were 0.5 ppm in urine and serum samples and 5 ppm in rumen contents. The diagnostic utility of the method was tested by analyzing samples from heifers dosed with blue oak leaves and commercial tannic acid. Keywords: Pyrogallol; gallic acid; gallotannins; GC/MS determination
Bibliography:L74
9629292
Abstract published in Advance ACS Abstracts, April 15, 1996.
istex:AA7243FCAEDC102DF0F4BCA939C727B2C0753154
ark:/67375/TPS-ZNW2PHNF-4
ISSN:0021-8561
1520-5118
DOI:10.1021/jf950238k