Magnetic Fe2O3−Polystyrene/PPy Core/Shell Particles:  Bioreactivity and Self-Assembly

• Published in: Langmuir Vol. 23; no. 22; pp. 10940 - 10949
• Main Authors: Mangeney, Claire, Fertani, Meriem, Bousalem, Smain, Zhicai, Ma, Ammar, Souad, Herbst, Fréderic, Beaunier, Patricia, Elaissari, Abdelhamid, Chehimi, Mohamed M
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 23.10.2007
• Subjects: Biocompatible Materials - chemical synthesis; Biocompatible Materials - chemistry; Chemistry; Coated Materials, Biocompatible; Colloidal state and disperse state; Electrochemistry; Exact sciences and technology; Ferric Compounds - chemistry; General and physical chemistry; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Microspheres; Nanoparticles - chemistry; Nanoparticles - ultrastructure; Physical and chemical studies. Granulometry. Electrokinetic phenomena; Polymers - chemistry; Polystyrenes - chemistry; Pyrroles - chemistry; Spectroscopy, Fourier Transform Infrared; Surface physical chemistry; Self assembly; Particle size; Nanoparticle; Glass; Activation; Copolymerization; X ray; Superparamagnetism; Cyclic voltammetry; Ester; Synthesis; Carboxylic acid; Coated particle; Carboxyl group; Photoelectron spectrometry; Styrene polymer; Chemical composition; Magnetic field; Diameter; Magnetic properties; Microsphere; Transition element compounds; Biotin; Latex; Plate; Electrochemical properties; Transmission electron microscopy; Morphology; Models; Pyrrole polymer
• ISSN: 0743-7463; 1520-5827
• DOI: 10.1021/la700492s

This paper describes the synthesis of new magnetic, reactive polystyrene/polypyrrole core/shell latex particles. The core consists of a polystyrene microsphere containing γ-Fe2O3 superparamagnetic nanoparticles (PSmag), and the shell is made of reactive N-carboxylic acid-functionalized polypyrrole (PPyCOOH). These PSmag−PPyCOOH latex particles, average diameter 220 nm, were prepared by copolymerization of pyrrole (Py) and the active carboxyl-functionalized pyrrole (PyCOOH) in the presence of PSmag particles. PNVP was used as a steric stabilizer. The functionalized polypyrrole-coated PSmag particles were characterized in terms of their particle size, surface morphology, chemical composition, and electrochemical and magnetic properties using transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), cyclic voltammetry, and SQUID magnetometry. Activation of the particle surface carboxyl groups was achieved using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS), which helps transform the carboxyl groups into activated ester groups (NSE). The activated particles, PSmag−PPyNSE, were further evaluated as bioadsorbents of biotin used as a model biomolecule. It was shown that biotin was immobilized at the surface of the PSmag−PPyNSE particles by forming interfacial amide groups. The assemblies of PSmag−PPyCOOH particles on glass plates were further investigated. When no magnetic field is applied, the particles assemble into 3D colloidal crystals. In contrast, under a magnetic field, one-particle-thick chains gathered in hedgehog-like architectures are obtained. Furthermore, PSmag−PPyCOOH coated ITO electrodes were shown to be electroactive and electrochemically stable, thus offering potentialities for creating novel high-specific-area materials for biosensing devices where the conducting polymer component would act as the transducer through its conductive properties.