Rapid Detection of Immunity Against Bacteria in Asian Honeybee and Western Honeybee with Quantification of Royalisin in the Hemolymphe by Fast ELISA

• Published in: Journal of agricultural and food chemistry Vol. 62; no. 38; pp. 9305 - 9309
• Main Authors: Shen, Li-Rong, Dilireba, Shatar, Zhou, Wen-Xiu, Wang, Yi-Ran, Li, Mei-Lu, Zhai, Liang
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 24.09.2014; American Chemical Society, Books and Journals Division
• Subjects: Animals; bacteria; Bacteria - immunology; Bees - chemistry; Bees - classification; Bees - immunology; Bees - microbiology; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Hemolymph - chemistry; Hemolymph - immunology; honey bees; immune response; Immunity; innate immunity; pathogens; polyclonal antibodies; Proteins - analysis; Proteins - immunology; rapid methods; royal jelly; immunity against bacteria; royalisin; rapid detection; honeybee; ELISA
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf501637g

Royalisin from royal jelly (RJ) is a valuable peptide both for the prevention of honeybee diseases and for RJ preservation. ELISA for fast determination of royalisin content in hemolymphe (RCH) of honeybees with polyclonal antibody against recombinant royalisin from Asian honeybee was established. Assay on RCHs of health samples from Asian honeybee and Western honeybee showed the former (7.06 μg/mL) was significantly higher than that of the latter (5.64 μg/mL, p < 0.01). Moreover, relative to the non infection, the RCHs of Asian honeybees at 24 and 48 h post infection of Eschericha coli were higher than those of Western honeybees by 32.90% and 29.66%, respectively. Evidence revealed that Asian honeybee possesses higher innate immunity and immune response against bacteria in relation to the Western honeybee. The method will be a potential tool for detection of resistant levels to pathogens in honeybees and for quantification of royalisin in RJ products.