In Vitro Adsorption of Aluminum by an Edible Biopolymer Poly(γ-glutamic acid)

• Published in: Journal of agricultural and food chemistry Vol. 62; no. 20; pp. 4803 - 4811
• Main Authors: Rajan, Yesudoss Christu, Inbaraj, Baskaran Stephen, Chen, Bing Huei
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 21.05.2014; American Chemical Society, Books and Journals Division
• Subjects: Adsorption; aluminum; Aluminum - chemistry; Aluminum - metabolism; aluminum hydroxide; Alzheimer disease; aqueous solutions; biopolymers; Biopolymers - chemistry; Biopolymers - metabolism; calcium; copper; Digestion; gastrointestinal system; Humans; Hydrogen-Ion Concentration; ionic strength; iron; Kinetics; Models, Biological; Parkinson disease; Polyglutamic Acid - analogs & derivatives; Polyglutamic Acid - chemistry; Polyglutamic Acid - metabolism; potassium; sorption isotherms; temperature; Thermodynamics; zinc; essential metals; gastrointestinal pH; aluminum; isotherms; detoxification; kinetics; poly(γ-glutamic acid)
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf5011484

Accumulation of aluminum in human has been reported to be associated with dementia, Parkinson’s disease, and Alzheimer’s disease. The objectives of this study were to evaluate an edible biopolymer poly(γ-glutamic acid) (γ-PGA) for aluminum removal efficiency under in vitro conditions as affected by pH, contact time, aluminum concentration, temperature, ionic strength, and essential metals in both aqueous aluminum solution and simulated gastrointestinal fluid (GIF). A low aluminum adsorption occurred at pH 1.5–2.5, followed by a maximum adsorption at pH 3.0–4.0 and precipitating thereafter as aluminum hydroxide at pH > 4. Adsorption was extremely fast with 81–96% of total adsorption being attained within 1 min, reaching equilibrium in 5–10 min. Kinetic data at low (10 mg/L) and high (50 mg/L) concentrations were well described by pseudo-first-order and pseudo-second-order models, respectively. Equilibrium adsorption isotherms at different temperatures were precisely fitted by both Langmuir and Redlich–Peterson models with the maximum adsorption capacities at 25, 37, and 50 °C being 35.85, 38.68, and 44.23 mg/g, respectively. Thermodynamic calculations suggested endothermic and spontaneous nature of aluminum adsorption by γ-PGA with increased randomness at the solid/solution interface. Variation in ionic strengths did not alter the adsorption capacity, however, the incorporation of essential metals significantly reduced the aluminum adsorption by following the order copper > iron > zinc > calcium > potassium. Compared to aqueous solution, the aluminum adsorption from simulated GIF was high at all studied pH (1–4) with Langmuir monolayer adsorption capacity being 49.43 mg/g at 37 °C and pH 4. The outcome of this study suggests that γ-PGA could be used as a safe detoxifying agent for aluminum.