Characterization of Selenium-Enriched Mycelia of Catathelasma ventricosum and Their Antihyperglycemic and Antioxidant Properties

• Published in: Journal of agricultural and food chemistry Vol. 63; no. 2; pp. 562 - 568
• Main Authors: Liu, Yuntao, Li, Caiming, Luo, Xiaohu, Han, Guoquan, Xu, Shude, Niu, Fuge, Hu, Xinjie, Wu, Hejun, Zhang, Huimin
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 21.01.2015; American Chemical Society, Books and Journals Division
• Subjects: Agaricales - chemistry; alpha-amylase; alpha-glucosidase; animal disease models; Animals; antioxidant activity; antioxidants; Antioxidants - administration & dosage; Antioxidants - chemistry; blood glucose; Blood Glucose - metabolism; body weight; diabetes mellitus; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - metabolism; dietary supplements; enzyme activity; functional foods; glycemic effect; Humans; hydrolysis; Hypoglycemic Agents - administration & dosage; Hypoglycemic Agents - chemistry; ingredients; insulin; Insulin - metabolism; Male; Mice; Mice, Inbred ICR; molecular weight; mycelium; Mycelium - chemistry; selenium; Selenium Compounds - administration & dosage; Selenium Compounds - chemistry; selenocysteine; selenomethionine; selenoproteins; spectroscopy; selenium speciation; mushroom; antioxidant activity; chemical composition; antihyperglycemic activity
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf5050316

This is the first report concerning the selenium enrichment of Catathelasma ventricosum mycelia. The selenium-containing proteins present in selenium-enriched mycelia (Se-MC) were identified using size-exclusion chromatography–inductively coupled plasma–mass spectrometry (SEC-ICP-MS). The selenium-containing amino acids liberated by hydrolysis of these proteins were identified using anion exchange-ICP-MS. Se-MC was found to contain selenoproteins with molecular weights ranging from 1.7 to 60.5 kDa. The main selenium-containing amino acids within them were selenomethionine and selenocysteine. Furthermore, Se-MC possessed excellent antihyperglycemic and antioxidant properties. Se-MC normalized biochemical parameters like insulin level, blood glucose level, body weight, and antioxidant enzyme activity in streptozocin-induced diabetic mice. It also inhibited the α-amylase and α-glucosidase activities present in in vitro gastric and intestinal models. In conclusion, Se-MC has the potential to serve as a dietary supplement of selenium, an antioxidant, or an ingredient for the formulation of nutraceuticals.