Production of Phytochromes by High-Cell-Density E. coli Fermentation

Phytochromes are important photoreceptors of plants, bacteria, and fungi responsive to light in the red and far-red spectrum. For increasing applications in basic research, synthetic biology, and materials sciences, it is required to recombinantly produce and purify phytochromes in high amounts. An...

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Published inACS synthetic biology Vol. 8; no. 10; pp. 2442 - 2450
Main Authors Hörner, Maximilian, Gerhardt, Karl, Salavei, Pavel, Hoess, Philipp, Härrer, Daniel, Kaiser, Johannes, Tabor, Jeffrey J, Weber, Wilfried
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 18.10.2019
Subjects
fermentation
phytochromes
PhyB
Cph1
E. coli
optogenetics
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Summary:Phytochromes are important photoreceptors of plants, bacteria, and fungi responsive to light in the red and far-red spectrum. For increasing applications in basic research, synthetic biology, and materials sciences, it is required to recombinantly produce and purify phytochromes in high amounts. An ideal host organism for this purpose is E. coli due to its widespread use, fast growth, and ability for high-cell-density fermentation. Here, we describe the development of a generic platform for the production of phytochromes in E. coli that is compatible with high-cell-density fermentation. We exemplify our approach by the production of the photosensory domains of phytochrome B (PhyB) from A. thaliana and of the cyanobacterial phytochrome 1 (Cph1) from Synechocystis PCC 6803 in the multigram scale per 10 L fermentation run.
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ISSN:2161-5063
2161-5063
DOI:10.1021/acssynbio.9b00267