Antibiotic-Induced Recruitment of Specific Algae-Associated Microbiome Enhances the Adaptability of Chlorella vulgaris to Antibiotic Stress and Incidence of Antibiotic Resistance

• Published in: Environmental science & technology Vol. 57; no. 36; pp. 13336 - 13345
• Main Authors: Xue, Xue, Su, Xiaoyue, Zhou, Linjun, Ji, Jiaqi, Qin, Ziwei, Liu, Jialin, Li, Kaiqi, Wang, He, Wang, Zaizhao
• Format: Journal Article
• Language: English
• Published: Easton American Chemical Society 12.09.2023
• Subjects: Adaptability; Algae; Algal growth; Antibiotic resistance; Antibiotics; Bacteria; Chlorella; Chlorella vulgaris; Consortia; Drug resistance; Ecotoxicology and Public Health; Germfree; Microbiomes; Phytoplankton; Recruitment; rRNA 16S; algae communities; algae-associated microbiome; antibiotic contamination; antibiotic adaptation; antibiotic resistance genes
• ISSN: 0013-936X; 1520-5851
• DOI: 10.1021/acs.est.3c02801

Insights into the symbiotic relation between eukaryotic hosts and their microbiome lift the curtain on the crucial roles of microbes in host fitness, behavior, and ecology. However, it remains unclear whether and how abiotic stress shapes the microbiome and further affects host adaptability. This study first investigated the effect of antibiotic exposure on behavior across varying algae taxa at the community level. Chlorophyta, in particular Chlorella vulgaris, exhibited remarkable adaptability to antibiotic stress, leading to their dominance in phytoplankton communities. Accordingly, we isolated C. vulgaris strains and compared the growth of axenic and nonaxenic ones under antibiotic conditions. The positive roles of antibiotics in algal growth were apparent only in the presence of bacteria. Results of 16S rRNA sequencing further revealed that antibiotic challenges resulted in the recruitment of specific bacterial consortia in the phycosphere, whose functions were tightly linked to the host growth promotion and adaptability enhancement. In addition, the algal phycosphere was characterized with 47-fold higher enrichment capability of antibiotic resistance genes (ARGs) than the surrounding water. Under antibiotic stress, specific ARG profiles were recruited in C. vulgaris phycosphere, presumably driven by the specific assembly of bacterial consortia and mobile genetic elements induced by antibiotics. Moreover, the antibiotics even enhanced the dissemination potential of the bacteria carrying ARGs from the algal phycosphere to broader environmental niches. Overall, this study provides an in-depth understanding into the potential functional significance of antibiotic-mediated recruitment of specific algae-associated bacteria for algae adaptability and ARG proliferation in antibiotic-polluted waters.