Polyphenolic Profiles of Yellow Bean Seed Coats and Their Relationship with Iron Bioavailability

• Published in: Journal of agricultural and food chemistry Vol. 68; no. 3; pp. 769 - 778
• Main Authors: Hart, Jonathan J, Tako, Elad, Wiesinger, Jason, Glahn, Raymond P
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 22.01.2020
• Subjects: Biological Availability; Biological Transport; Caco-2 Cells; Humans; Iron - chemistry; Iron - metabolism; Phaseolus - chemistry; Phaseolus - classification; Phaseolus - metabolism; Plant Extracts - chemistry; Plant Extracts - metabolism; Polyphenols - chemistry; Polyphenols - metabolism; Seeds - chemistry; Seeds - metabolism; Caco-2 cells; iron bioavailability; polyphenol composition; kaempferol 3-glucoside; yellow bean seed coats
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.9b05663

Previous work with Caco-2 cell cultures has shown that individual polyphenols can either promote or inhibit iron uptake. This investigation was designed to characterize the relationship between iron bioavailability and seed coat polyphenol composition in a panel of 14 yellow beans representing five market classes with the potential for fast cooking time and high iron content. The study included two white and two red mottled bean lines, which represent high and low iron bioavailability capacity in dry beans, respectively. Polyphenols were measured quantitatively by high-performance liquid chromatography–mass spectrometry (HPLC–MS)/UV and iron bioavailability of seed coat extracts was measured in Caco-2 assays. Thirteen of the yellow bean seed types contained high concentrations (up to 35.3 ± 2.7 μmol/g) of kaempferol 3-glucoside (k 3-g), a known promoter of iron uptake. A general association between the ratio of promoting to inhibiting polyphenols (P/I) and iron uptake was observed. The presence of iron uptake inhibiting condensed tannins proportionately countered the promotional effects of kaempferol compounds. Unidentified factors present in seed coats other than polyphenols also appeared to affect iron uptake.