Effects of Condensed and Hydrolyzable Tannins on Rumen Metabolism with Emphasis on the Biohydrogenation of Unsaturated Fatty Acids

• Published in: Journal of agricultural and food chemistry Vol. 66; no. 13; pp. 3367 - 3377
• Main Authors: Costa, Mónica, Alves, Susana P, Cappucci, Alice, Cook, Shaun R, Duarte, Ana, Caldeira, Rui M, McAllister, Tim A, Bessa, Rui J. B
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 04.04.2018
• Subjects: condensed tannins; fatty acids; biohydrogenation; hydrolyzable tannins; rumen
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.7b04770

The hypothesis that condensed tannins have higher inhibitory effect on ruminal biohydrogenation than hydrolyzable tannins was tested. Condensed tannin extract from mimosa (CT) and hydrolyzable tannin extract from chestnut (HT) or their mixture (MIX) were incorporated (10%) into oil supplemented diets and fed to rumen fistulated sheep. Fatty acid and dimethyl acetal composition of rumen contents and bacterial biomass were determined. Selected rumen bacteria were analyzed by quantitative real time PCR. Lower (P < 0.05) rumen volatile fatty acids concentrations were observed with CT compared to HT. Moreover, lower concentration (P < 0.05) of Fibrobacter succinogenes, Ruminococcus flavefaciens, Ruminococcus albus, and Butyrivibrio proteoclasticus were observed with CT compared to HT. The extension of biohydrogenation of 18:2n-6 and 18:3n-3 did not differ among treatments but was much more variable with CT and MIX than with HT. The trans-/cis-18:1 ratio in bacterial biomass was higher (P < 0.05) with HT than CT. Thus, mimosa condensed tannins had a higher inhibitory effect on ruminal metabolism and biohydrogenation than chestnut hydrolyzable tannins.