Utilization of Soybean Oil Waste for a High-Level Production of Ceramide by a Novel Phospholipase C as an Environmentally Friendly Process

• Published in: Journal of agricultural and food chemistry Vol. 70; no. 10; pp. 3228 - 3238
• Main Authors: Wang, Fenghua, Guo, Zehui, Yang, Zixuan, Li, Xueying, Zhang, Xue, Ma, Xiangyang, Han, Zhuoxuan, Lu, Fuping, Liu, Yihan
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 16.03.2022
• Subjects: Biotechnology and Biological Transformations; Ceramides; Soybean Oil - chemistry; Sphingomyelins - metabolism; Type C Phospholipases - metabolism; phospholipase C; ceramide; Acinetobacter calcoaceticus; crude soybean oil sediment waste; whole-cell biocatalyst
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.1c08362

Ceramide is a natural functional ingredient as food additive and medicine that has attracted extensive attention in the food, medical, and cosmetic industries. Here, we developed a biotechnological strategy based on a recombinant whole-cell biocatalyst for efficiently producing ceramide from crude soybean oil sediment (CSOS) waste. A novel phospholipase C (PLCac) from Acinetobacter calcoaceticus isolated from soil samples was identified and characterized. Furthermore, recombinant Komagataella phaffii displaying PLCac (dPLCac) on the cell surface was constructed as a whole-cell biocatalyst with better thermostability (30–60 °C) and pH stability (8.0–10.0) to successfully produce ceramide. After synergistical optimization of reaction time and dPLCac dose, the ceramide yield of hydrolyzing from CSOS using dPLCac was 51% (the theoretical maximum yield of converting sphingomyelin, ∼70%) and the relative yield was over 50% after seven consecutive 4 h batches under the optimized conditions. Our study provides a potentially promising strategy for the commercial production of ceramide.