Comparative Proteomic and Nutritional Composition Analysis of Independent Transgenic Pigeon Pea Seeds Harboring cry1AcF and cry2Aa Genes and Their Nontransgenic Counterparts

• Published in: Journal of agricultural and food chemistry Vol. 65; no. 7; pp. 1395 - 1400
• Main Authors: Mishra, Pragya, Singh, Shweta, Rathinam, Maniraj, Nandiganti, Muralimohan, Ram Kumar, Nikhil, Thangaraj, Arulprakash, Thimmegowda, Vinutha, Krishnan, Veda, Mishra, Vagish, Jain, Neha, Rai, Vandna, Pattanayak, Debasis, Sreevathsa, Rohini
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 22.02.2017
• Subjects: Amino Acids - analysis; Amino Acids - metabolism; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Cajanus - chemistry; Cajanus - genetics; Cajanus - metabolism; Endotoxins - genetics; Endotoxins - metabolism; Hemolysin Proteins - genetics; Hemolysin Proteins - metabolism; Minerals - analysis; Minerals - metabolism; Nutritive Value; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified - chemistry; Plants, Genetically Modified - genetics; Proteomics; Seeds - chemistry; Seeds - genetics; Seeds - metabolism; pigeon pea; transgenics; nutritional analysis; proteome
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.6b05301

Safety assessment of genetically modified plants is an important aspect prior to deregulation. Demonstration of substantial equivalence of the transgenics compared to their nontransgenic counterparts can be performed using different techniques at various molecular levels. The present study is a first-ever comprehensive evaluation of pigeon pea transgenics harboring two independent cry genes, cry2Aa and cry1AcF. The absence of unintended effects in the transgenic seed components was demonstrated by proteome and nutritional composition profiling. Analysis revealed that no significant differences were found in the various nutritional compositional analyses performed. Additionally, 2-DGE-based proteome analysis of the transgenic and nontransgenic seed protein revealed that there were no major changes in the protein profile, although a minor fold change in the expression of a few proteins was observed. Furthermore, the study also demonstrated that neither the integration of T-DNA nor the expression of the cry genes resulted in the production of unintended effects in the form of new toxins or allergens.