Novel Colorimetric Immunoassay for Ultrasensitive Monitoring of Brevetoxin B Based on Enzyme-Controlled Chemical Conversion of Sulfite to Sulfate
A simple colorimetric immunoassay for quantitative monitoring of brevetoxin B on a functionalized magnetic bead by using glucose oxidase (GOx)/antibrevetoxin antibody-labeled gold nanoparticle as the signal transduction tag was developed. The assay was carried out on the basis of GOx-controlled sulf...
Saved in:
Published in | Journal of agricultural and food chemistry Vol. 63; no. 7; pp. 1982 - 1989 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
American Chemical Society
25.02.2015
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | A simple colorimetric immunoassay for quantitative monitoring of brevetoxin B on a functionalized magnetic bead by using glucose oxidase (GOx)/antibrevetoxin antibody-labeled gold nanoparticle as the signal transduction tag was developed. The assay was carried out on the basis of GOx-controlled sulfite-to-sulfate chemical conversion with a silver(I)–3,3′,5,5′-tetramethylbenzidine [Ag(I)–TMB] system. Initially, the sulfite was used as an inhibitor of Ag(I) to hinder the color development of TMB due to the formation of insoluble silver sulfite. Accompanying H2O2 generation with GOx-catalyzed glucose, the sulfite was converted into the sulfate, thus resulting in the colorless-to-blue change. Under the optimal conditions, the absorbance decreased with increasing brevetoxin B from 0.5 to 200 ng/kg with a detection limit of 0.1 ng/kg (ppt). The precision and specificity were acceptable. Furthermore, the methodology gave results matching well with the referenced brevetoxin ELISA kit for monitoring of spiked Musculista senhousia samples. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0021-8561 1520-5118 |
DOI: | 10.1021/acs.jafc.5b00425 |