Luminol/H2O2 Chemiluminescence Detector for the Analysis of Nitric Oxide in Exhaled Breath

• Published in: Analytical chemistry (Washington) Vol. 71; no. 22; pp. 5131 - 5136
• Main Authors: Robinson, Jill K, Bollinger, Mark J, Birks, John W
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 15.11.1999
• Subjects: Analytical biochemistry: general aspects, technics, instrumentation; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Breath Tests; Calibration; Chemistry; Fundamental and applied biological sciences. Psychology; Gases; Humans; Hydrogen Peroxide; Instruments; Luminescent Measurements; Luminol; Nitric Oxide - analysis; Pollutants; Respiratory system; Expired air; Analysis method; Nitric oxide; Measurement sensor; Instrumentation; Chemiluminescence; Quantitative analysis
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac990646d

A new instrument for the detection of nitric oxide has been developed and applied to the analysis of exhaled breath. The instrument is based on conversion of NO to NO2, using the oxidant chromium trioxide, followed by detection of chemiluminescence in the reaction of NO2 with an alkaline luminol/H2O2 solution. The presence of H2O2 is found to enhance the sensitivity of NO2 detection by a factor of ∼20. A bundle of porous polypropylene hollow fiber membranes is used to bring the gaseous sample into contact with the luminol solution. Chemiluminescence occurring within the translucent hollow fibers is detected using a miniature photomultiplier tube. The limit of detection for NO is 0.3 ppbv for S/N = 3, and the 1/e response time is 2 s. A large interference resulting from the 4−6% CO2 concentration in exhaled breath is removed by use of an ascarite scrubber in the air stream. Breath measurements of NO were made using a sampling technique developed by Sensor Medics (Yorba Linda, CA) with simultaneous detection using the luminol/H2O2 and NO + O3 chemiluminescence techniques. The two instruments were found to be in excellent agreement. Nitric oxide levels were in the range 6.0−22.0 ppbv for healthy individuals and 40.0−80.0 ppbv for individuals with asthma or a respiratory infection. This new detector offers the advantages of compact size, low cost, and a simple configuration compared to NO detectors based on NO + O3 chemiluminescence.