Surface Multifunctionalization of Inert Ceramic Implants by Calcium Phosphate Biomimetic Coating Doped with Nanoparticles Encapsulating Antibiotics

• Published in: ACS applied materials & interfaces Vol. 15; no. 17; pp. 21699 - 21718
• Main Authors: Desante, Gaëlle, Pudełko, Iwona, Krok-Borkowicz, Małgorzata, Pamuła, Elżbieta, Jacobs, Philipp, Kazek-Kęsik, Alicja, Nießen, Jonas, Telle, Rainer, Gonzalez-Julian, Jesus, Schickle, Karolina
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 03.05.2023
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Biomimetics; Calcium Phosphates - chemistry; Calcium Phosphates - pharmacology; Ceramics - pharmacology; Coated Materials, Biocompatible - chemistry; Coated Materials, Biocompatible - pharmacology; Escherichia coli; Humans; Nanoparticles; Osteogenesis; Staphylococcus aureus; Surface Properties; Surfaces, Interfaces, and Applications; Titanium - chemistry; coprecipitation; nanoparticles (NPs); biomimetic calcium phosphate coating; zirconia implant; drug delivery system (DDS)
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.3c03884

Aseptic loosening and periprosthetic infections are complications that can occur at the interface between inert ceramic implants and natural body tissues. Therefore, the need for novel materials with antibacterial properties to prevent implant-related infection is evident. This study proposes multifunctionalizing the inert ceramic implant surface by biomimetic calcium phosphate (CaP) coating decorated with antibiotic-loaded nanoparticles for bioactivity enhancement and antibacterial effect. This study aimed to coat zirconium dioxide (ZrO2) substrates with a bioactive CaP-layer containing drug-loaded degradable polymer nanoparticles (NPs). The NPs were loaded with two antibiotics, gentamicin or bacitracin. The immobilization of NPs happened by two deposition methods: coprecipitation and drop-casting. X-ray diffraction (XRD), scanning electron microscopy (SEM), and cross-section analyses were used to characterize the coatings. MG-63 osteoblast-like cells and human mesenchymal stem cells (hMSC) were chosen for in vitro tests. Antibacterial activity was assessed with S. aureus and E. coli. The coprecipitation method allowed for a favorable homogeneous distribution of the NPs within the CaP coating. The CaP coating was constituted of hydroxyapatite and octacalcium phosphate; its thickness was 3.8 ± 1 μm with cavities of around 1 μm suitable for hosting NPs with a size of 200 nm. Antibiotics were released from the coatings in a controlled manner for 1 month. The cell culture study has confirmed the excellent behavior of the coprecipitated coating, showing cytocompatibility and a homogeneous distribution of the cells on the coated surfaces. The increase in alkaline phosphatase activity showed osteogenic differentiation. The materials were found to inhibit the growth of bacteria. Newly developed coatings with antibacterial and bioactive properties are promising candidates to prevent peri-implant infectious bone diseases.